BD FACSAria™ Fusion Cell Sorter
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BD FACSAria Fusion Cell Sorter ™ The fusion of safety, performance and sorting

The fusion of safety, performance, and sorting The BD FACSAria™ Fusion cell sorter improves on the solid foundation of patented technologies, exceptional multicolor performance, and ease-of-use that was first brought to the world of sorting by the launch of the BD FACSAria™ cell sorter in 2003. Now this sorting know-how is combined with best-in-class biosafety expertise to create the BD FACSAria Fusion, a fully integrated advanced cell sorter and biosafety solution for research laboratories. Biological safety in flow cytometry is an emerging requirement for core laboratories concerned about...

Proven dependability and ease-of-use put the system in a class of its own Sensitivity for multicolor and sorting applications From its fixed alignment flow cell, fixed optical architecture, to software-controlled setup and operation, the BD FACSAria Fusion streamlines sorting and analysis. Gel-coupled cuvette flow cell At the heart of the BD FACSAria Fusion is the patented nextgeneration quartz cuvette flow cell that is in true fixed alignment with the laser, and is gel coupled to the collection optics. This patented design helps ensure that lasers are precisely focused on the sample stream...

Aligns with biosafety support Fixed alignment of the flow cell, and software control of the system also reduce the frequency of operator interaction with the instrument, providing an additional level of biosafety support. Sensitivity to resolve dim staining populations Enhancements to the BD FACSAria Fusion optical system take fluorescence sensitivity to an even higher level. Specifically selected for optimal signal-to-noise separation, the new lasers improve the resolution of dim populations, thereby enabling more effective gating for sorting. Set at peak performance, the lasers enable dim...

Fluidic system improvements are built-in for easier, safer operation Engineered into the system Easily accessible fluids and waste container Sheath and cleaning fluid tanks, as well as waste collection, are located in an easy to reach drawer at the base of the system. This includes a 10-L stainless steel sheath tank and a 10-L waste container. The sheath tank can be autoclaved. In addition, the drawer holds three 5-L auxiliary cleaning fluid containers used in conjunction with the automated Prepare for Aseptic Sort mode and instrument shutdown. An auxiliary air input is designed to connect...

From the sort block to the collection chamber An enclosed pathway from the sample injection chamber to the sort collection tubes isolates aerosols inside the sort collection chamber. The sort block design fixes deflection plates in position for more efficient and reproducible deflection into a collection device in the sort collection chamber. The sort block also houses an aspirator drawer that keeps the sort collection tubes covered until sorting begins and automatically closes to protect the tubes when the Sweet Spot monitoring system is on and a clog is detected. The sort collection...

Sensitivity and resolution beyond compare Increased efficiency for multicolor detection Innovations in the optical system, pioneered by BD, efficiently maximize signal detection and greatly increase sensitivity and resolution for each color in a multicolor assay. Enhanced sensitivity and resolution mean that even dim populations can be readily identified and sorted. The optics system allows optimizing multicolor assays and panel design for superior results. The design provides researchers a choice of laser excitation wavelength(s) that illuminate cells in the sample. Collection optics...

Octagon detector arrays This design is based on the principle that light reflection is more efficient than light transmission. Emitted light travels to each PMT via reflection and is transmitted through only two pieces of glass to reach each detector. Therefore, colors can be detected with minimum light loss. Precision optical design The many innovations in the BD FACSAria Fusion’s optical system, such as the patented gel-coupled cuvette and octagon detection system, and the 9-μm x 65-μm beam spot, are designed to work together to maximize sensitivity and resolution. This precision design...

Flexible for present and future needs For many users, the capability of an advanced cell sorter is defined by its flexibility, which in turn is defined by the number of parameters that can be detected simultaneously. With flexibility built in, the BD FACSAria Fusion can support six lasers and four spatially separated beam spots. Choose up to six laser wavelengths and up to 20 detector positions, to measure up to 18 colors simultaneously. Upgradable and backward compatible Users can design a configuration that meets their lab’s budget and site requirements today and have a growth path for...

X-mount optical plate Tube: mouse BM CD34KSL Hoechst 5 *0ug/ml Population All Events Cells PI- #Events %Parent %Total Doublet Doublet-CD45+ Lineagec-Kit+ Sca-1 + LinSide population c-Kit+ Sca-1 + Lin- AND Side population Tube: HT-29 Hoechst 5ug/ml Population All Events Cells FSC DoubletSSC DoubletPISide Population G0/G1 G2/M As a control, side population expression was blocked, as represented in the histogram. Cancer cell line side population As shown in the dot plot, human HT-29 colon cancer cells were stained with Hoechst 33342 and acquired on a BD FACSAria III equipped with a 375-nm...

BD FACSDiva software from analysis to sorting Compatible with other BD analyzers and sorters The BD FACSAria Fusion uses BD FACSDiva software to efficiently control the setup, acquisition, and analysis of flow cytometry data from the operator workstation. Software control of key operations minimizes direct interaction with the sorter for an added level of biosafety protection. BD FACSDiva software is common across many BD cell analyzers and cell sorters, including BD FACSCanto and BD LSRFortessa systems. Researchers gain application flexibility because it is easier to move the assay design...