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Atlas of Stains - 120 Pages

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Catalogue excerpts

FLEX Ready-to-Use Atlas of Stains - 4th Edition Cancer Diagnostics

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Committed to raising the bar for higher quality During the last decades, organizations such as CAP, UK NEQAS and NordiQC have successfully implemented many initiatives to improve standardization in immunohistochemistry. At the same time, laboratories are striving to deliver accurate clinical diagnostic results while facing and adapting to the increasing number of challenges. It is vital for pathology laboratories to make continuous improvements to maintain the quality of diagnoses, and to be a professional counterpart and key resource to oncologists, and other clinicians. Pathology laboratories...

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Ensuring desired staining performance with the help of an expert panel The FLEX RTU concept was launched in 2008, and the staining performance of the products was developed in collaboration with leading pathologists and their laboratory managers. In the process of ensuring desired staining performance, a panel of these distinguished anatomic p ­ athology experts specified the relevant criteria and reviewed the staining results obtained during development of all the antibodies. During a year-long process, the expert panel specified the required staining performance for each individual antibody....

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FLEX Ready-to-Use During the past 40 years, immunohistochemistry has become increasingly important in diagnostic pathology, and is now an essential daily tool for cancer diagnosis in most laboratories worldwide. This has led to intense focus on utilizing and expanding IHC for purposes such as implementation of new markers, use of established markers in new areas, and optimization of immunohistochemical techniques. However, IHC is technically complex, and determining what to choose in order to deliver the right results is a major challenge for laboratories. The fact is that there are more than...

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How to read the Atlas of Stains The diagnostic staining performance delivered by the Dako FLEX Ready-to Use (RTU) system has been accep­ed by t leading experts in the field and worked out in collaboration with Søren Nielsen, Scheme Organizer at NordiQC. The Dako FLEX RTU procedure makes it possible to deliver a high quality staining performance in all relevant clinical tissues. Information on the antibodies has been organized under the following four headings: n n n n Clinical Application Reaction Location Recommended Control Differential Diagnosis The Atlas of Stains illustrates the staining...

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The code IR/IS refers to: IR = FLEX ready-to-use antibodies for Autostainer Link instruments IS = FLEX ready-to-use antibodies for Dako Autostainer/Autostainer Plus instruments The basis for evaluating the quality of IHC performance is the use of proper controls. Benign tissue that is easily accessible and interpretable is recommended as control for most anti­ odies in the FLEX RTU system. Accompanying b photos illustrate the reaction pattern of the cell types and/or cellular structures which should be identified in the control tissue. These cell types and/or cellular structures should be considered...

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FLEX Ready-to-Use Actin (Muscle), Clone HHF35.. . . . . . . . . . . . . . . . . . . . . . . . . . . Alpha-1-Antitrypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Alpha-1-Fetoprotein. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . AMACR (P504S), Clone 13H4.. . . . . . . . . . . . . . . . . . . . . . . . . . Amyloid A, Clone mc1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-Cell-Specific Activator Protein, Clone DAK-Pax5. . . . . . . . BCL2 Oncoprotein, Clone 124. . . . . . . . . . . . . . . . . . . . . . . . . . BCL6 Protein,...

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Cytomegalovirus, Clone CCH2 + DDG9. . . . . . . . . . . . . . . . 63 D2-40, Clone D2-40. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Desmin, Clone D33. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 E-Cadherin, Clone NCH-38. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 Epithelial Antigen, Clone Ber-EP4.. . . . . . . . . . . . . . . . . . . . . . . 67 Epithelial Membrane Antigen, Clone E29. . . . . . . . . . . . . . . . . 68 Epstein-Barr Virus, LMP-1, Clone CS.1-4. . . . . . . . . . . . . . . 69 Ets-Related Gene (ERG), Clone EP111....

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Antibody: Monoclonal Mouse Anti-Human Actin (Muscle) Clone: HHF35 Code: IR700 or IS700 Clinical Application Reaction Location Recommended Control For identification of soft tissue tumors with muscle differentiation, i.e. leiomyoma, leiomyo­ sarcoma (Fig. A) and rhabdomyosarcoma (Fig. B). Cytoplasm. Appendix/Colon: All the smooth muscle cells in vessel walls, muscle layers and lamina muscularis mucosa should show a moderate to strong cytoplasmic staining reaction. The fine layer of myoepithelial cells delineating the surface epithelial cells should be demon­ strated. No staining reaction should...

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Antibody: Polyclonal Rabbit Anti-Human Alpha-1-Antitrypsin Code: IR505 or IS505 Clinical Application Reaction Location Recommended Control Primarily for identification of alpha-1-antitrypsin (A1AT) accumulation in the hepatocytes of A1AT deficient liver (Fig. A). Cytoplasm. Tonsil: The germinal center macrophages of the secondary follicles should show a moderate to strong staining reaction and neutrophil granulocytes should show a weak to moderate distinct coarse granular cytoplasmic staining reaction (Fig. B). Liver: The Kupffer cells should show a moderate to strong distinct granular staining...

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Antibody: Polyclonal Rabbit Anti-Human Alpha-1-Fetoprotein Code: IR500 or IS500 Clinical Application Reaction Location Recommended Control Differential Diagnosis* Primarily for identification of hepatocellular carcinoma, hepatoid tumor, and germ cell tumors of the testis and ovary such as yolk sac tumor (Fig. A) and embryonal carcinoma (Fig. B and Fig. C). Cytoplasm. Embryonal carcinoma: Focally, the neoplastic cells should show a moderate to strong cytoplasmic staining reaction with minimal background staining (Fig. B and Fig. C). 1. Yolk sac tumor vs. clear cell carcinoma. 2. Embryonal carcinoma...

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