Dynamic gas mixtures with adjustable O2 and CO2 concentration in cell cultures study
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High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface Application Note MCQ Gas Blender 100 Series Application: Dynamic gas mixtures with adjustable O2 and CO2 concentration in cell cultures study Introduction Starting from the pioneering studies made in the early 1900’s, the cell culture process has been developed and refined to become one of the most important and widely used experimental method for the scientific research. The growth of cells is a complex process that requires appropriate and controlled conditions (such as pressure, temperature, and system pH) to be achieved. A crucial role is carried out by the gas mixture fluxed over the culture. The instrument for the gas mixtures management must secure high precision outcomes over the whole experiement duration; high accuracy, great stability and high repeatability are required. On the other hand it’s also important to work with an instrument capable of creating gas mixtures dynamically adjustable since variation of the conditions for a particular cell type can result in gene/protein/enzyme expression changes. For all these applications we propose the use MCQ Gas Blender 100 Series, an instrument specifically made to work with 3 components gas mixtures that offers a quick and easy way to efficiently manage the experimental condition of your cell culture. for cell culture. In most of the common applications the cells are grown in an atmosphere of 5-10% CO2, inside special CO2 incubators. The basic CO2 incubators make use of gas mixture cylinders (pre-mixed by the supplier) in which the amount of CO2 in the flow cannot be changed or adjusted by the user. Newer and more advanced incubators include a CO2 sensor for a feedback regulation of the the amount of CO2 inside the chambers but are less common and more expansive then the basic ones. Dynamic gas mixtures applications Working with static gas mixtures with fixed or adjustable amount of CO2 represents now just the base of the cell culture applications. Since the last two decades until today many experiments have been successfully conducted with gas mixtures dynamically obtained and many more demands for instruments capable of controlling both O2 and CO2 relative amounts in the culture atmosphere. Fine control over the gas mixture allow those experiments, in which an induced hypoxic, hyperoxic or hypercapnic state is required, to be performed efficiently. Common experimental setup One of crucial aspect of the grown of cells in vitro is the complete control over the working parameters during the entire process. Even if the cell culture applications may vary widely from each other, growth medium represent one the most important of these parameter. The medium used is usually buffered with sodium bicarbonate/carbonic acid and for a proper cell grown the pH value must be strictly maintained constant. The amount of CO2 in the culture atmosphere directly affects the medium pH, therefore the control over the gas mixture in which cells are grown becomes another fundamental process parameter. Due to the low content of CO2 (<0,04%), the air isn’t a valid option www.mcqinst.com Gas Blender 100 Series Application Note

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface Dry air contains roughly 78.08% nitrogen, 20.95% oxygen, 0.92% argon, 0.04% carbon dioxide and other components in trace (mostly noble gases). An hypoxic gas mixture is by definition a mixture with a lower oxygen content compared with air. Basically the amount of O2 in a hypoxic mixture goes from a minimum of 0% (this condition is called anoxia) to a maximum of 21%. On the contrary a gas mixture that exceeds 21% oxygen is defined hyperoxic. Inducing hypoxic condition over the cell cultures is a common...

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface change (50ms). Along with the instrument a software for the gas mixture management, the MCQ Gas Mixer Manager, is also provided. The Gas Mixer Manager, manageable with any common desktop or laptop pc, has been designed to allow the user to take a complete control over the instrument and its functions. Easy and intuitive the Gas Mixer Manager allows to start working with dynamic mixtures immediately. have been chosen for simplicity). The gas cylinders are connected to the instrument through 6 mm diameter tubes and...

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface References [1] J. G. Murphy, T. W. Smith and J. D. Marsh, Calcium flux measurements during hypoxia in cultured heart cells – J. Mol. Cell. Cardiol. 19, 3 (1987) 271-279. [2] I. Ginis, D. V. Faller, Hypoxia affects tumor cell invasiveness in vitro: the role of hypoxia-activated ligand HAL1/13 (Ku86 autoantigen) – Cancer Lett. 154, 2 (2000) 163-174. [3] M. Weinmann, P. Marini, V. Jendrossek, A. Betsch, B. Goecke, W. Budach and C. Belka, Influence of hypoxia on TRAIL-induced apoptosis in tumor cells - Int. J....