FV3000
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Confocal Laser Scanning Microscope Next Generation FLUOVIEW for the Next Revolutions in Science

FLUOVIEW FV3000 Laser Scanning Microscope The FLUOVIEW FV3000 series is designed to meet some of the most difficult challenges in modern science. With the high sensitivity and speed required for live cell and tissue imaging and the ease of use and flexibility required for microplate imaging and complex screening protocols, the FV3000 series supports complete workflows from live cell 2D–6D (x,y,λ,z,t,p) imaging to image processing, such as deconvolution, and analysis. With Olympus’ renowned optics at the heart of the system, the FV3000 laser scanning microscope features TruSpectral detection...

Meeting Your Application Challenges Advanced Hardware High-Sensitivity Multiplex Imaging from Violet to NIR Macro-to-Micro Imaging and Super Resolution Accurate TimeLapse Imaging

Adapt the User Interface for Your Workflows • Select the layout appropriate for your experiment • Acquire multidimensional images • Reload the settings from previous experiments • Acquire multi-area images • Review images during or after acquisition using multiple display methods • Various image analysis menus are available Increase Productivity with High-Speed Imaging • Acquire images using a complex protocol Superior Objectives • X Line • A Line • One-click macro analysis Advanced Solutions • TruAI Deep Learning

Acquiring multicolor fluorescent images is important for analyzing the fine internal structure of cells and tissues and confirming protein expression. The FV3000 system's TruSpectral detectors enable users to select the detection wavelength on each channel to optimize signal detection for each individual fluorophore. The variable barrier filter mode provides simultaneous four-channel image acquisition, and up to 16 channels sequentially. The system's online or offline lambda scanning mode facilitates the separation of complex fluorescence signals by defining characteristic spectral emission...

Adjustable slit High-Sensitivity Multiplex Imaging Efficient TruSpectral Detection System The FV3000 series employs Olympus’ TruSpectral detection technology. Based on patented volume phase hologram (VPH) transmission and an adjustable slit to emitted light, the spectral detection is highly efficient, enabling users to select the detection wavelength of each individual channel at the spectral resolution of 1 nm. All the detectors in the FV3000 series have spectral imaging capability. TruSpectral detection delivers up to three-fold Transmittance Ratio of FV3000 sensitivity with a high...

Application: FV3000 Red System for NIR Solutions Near-infrared (NIR) fluorescence expands the possibilities for multiplexing, gentle live cell imaging, and deeper imaging, while at same time reducing autofluorescence. The FV3000 Red system's detection optical path greatly improves near-infrared transmittance. Moreover, it is equipped with NIR lasers, such as 730 nm or 785 nm wavelengths, and 1–2 channels of NIRdedicated GaAs detectors (~890 nm), which enable six-channel multiplexed imaging from 400 nm to 890 nm. Simultaneous imaging of up-conversion fluorescence of nanoparticies and...

Reflectance (%) (Quantum Efficiency(%) Wavelength (nm) - Multi-alkali PMT - GaAsP PMT - GaAs PMT GaAs photomultiplier tube (PMT) has a higher quantum efficiency at 700 nm-890 nm range compared with a conventional GaAsP PMT or mutli-alkali PMT. Wavelength (nm) ----DAPI----GFP----RFP----Alexa Fluor 647 ----Alexa Fluor 750 NIR laser diodes help reduce crosstalk caused by dye selection. These laser diodes (LD730/785) are designed to be driven in continuous wave (CW) mode, offering stability, low maintenance, and a longer lifetime. Efficient NIR imaging requires high-quality optics. All the...

Life science research applications require users to observe regions of interest within the context of the larger tissue structure. A large field of view is critical to being able to see a cell's components in relation to the entire tissue sample. The FV3000 microscope‘s optical light path facilitates macro-to-micro observation from 1.25X to 150X. Image stitching enhances these applications even more by enabling users to quickly locate target cells on the macro image and follow up with higher resolution imaging of the cell's fine structures. For even greater resolution, Olympus Super...

Macro-to-Micro Imaging and Super Resolution TruSight Deconvolution TruSight deconvolution image processing improves the sharpness and clarity of images by reducing optical blur and noise. The optimized image processing mode for optical characteristics enables the restoration of confocal images acquired using the FV3000 microscope. TruSight deconvolution can also reduce noise for Olympus Super Resolution (FV-OSR) acquired images. TruSight deconvolution uses a GPU for high-speed image processing. Without TruSight With TruSight cLSM image without TruSight With TruSight HeLa cell. Blue: nuclear...

Application: Time-Lapse Imaging Time-lapse imaging experiments require focus to be maintained throughout with less phototoxicity to the sample. Olympus’ TruFocus Z-drift compensator helps each position stay in focus during an experiment despite changes in temperature or the addition of reagents. Additionally, the FV3000 system’s high-sensitivity detector requires reduced laser intensity, and the resonant scanner reduces the laser illumination time, reducing phototoxicity for more physiologically accurate imaging data. 3D time-lapse imaging of a mouse embryonic fibroblast labeled with...

Accurate Time-Lapse Imaging Maintain Focus with TruFocus Z-Drift Compensation Stage Control for Multi-Area Time-Lapse, Microplate, and Stitching The TruFocus ZDC system uses minimally-phototoxic infrared light (laser class 1) to identify the location of the sample plane. One-shot autofocus (AF) mode enables several focus positions to be set as desired for thicker samples, enabling efficient Z-stack acquisitions in multiposition experiments. The continuous AF mode keeps the desired plane of observation precisely in focus, avoiding focus drift caused by temperature changes or the addition of...