IncuSafe Multigas Incubators - Understanding Biological Oxygen Levels - 4 Pages

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Catalogue excerpts

In April 2018, our Biomedical Business Brand will change to PHCbi IncuSafe Multigas Incubators Understanding Biological Oxygen Levels LOW OXYGEN TECHNICAL BULLETIN IncuSafe Multigas Incubators | MCO-170M In today’s life science research sector, there are a variety of ways to grow, or “culture” cells. Depending on the organism or cell of interest, different incubation parameters are required for cell cultivation. Mammalian cell culture, a method of growing a particular tissue type out of its natural environment (or in vitro), has been an extremely valuable tool for the progress of science and medicine. To keep these delicate cells alive and healthy, CO2 incubators are used to properly balance the temperature, pH level, and humidity of cells in vitro. Often overlooked, but very impactful to cellular health, is the level of oxygen at which mammalian cells are cultured. Multigas incubators are one of several tools that lower oxygen within an incubator or chamber to bring cultured cells the oxygen levels that precisely mimic the tissue from which a particular cell is derived. The descriptions utilized to describe the various levels of oxygen conditions within cell culture can get convoluted. “Normoxia”, “physiological normoxia” (physoxia or physiological oxygen), “hypoxia” (physiological and pathological hypoxia), and “anaerobia” are all examples of common terms used to describe atmospheric or sub-atmospheric oxygen levels. This document seeks to elucidate the exact meaning of these terms and what biological applications are most commonly used in each setting. “Normoxia” or Atmospheric Oxygen (approximately 20-21% O2) Normoxia is the term most often used to describe atmospheric levels of oxygen. This range generally resides between 20-21% O2 (160 mmHg)[1]. Oxygen levels can vary depending on the altitude as well as how much CO2 is being utilized within a given system [1]. Most cell culture today is performed at normoxic conditions with CO2 maintained at 5%. In these conditions, cells are exposed to atmospheric air, which has a concentration of generally 20-21% O2. Whereas, the majority of cell lines grown by cell culture are derived from tissues that physiologically contain oxygen levels of 12% or lower. High oxygen concentrations, in respect to in vivo conditions, correlate with a higher generation of reactive oxygen species, which can be damaging to DNA[2]. Normoxic studies are most frequently performed in CO2 incubators that do not have oxygen control. These incubators are able to maintain CO2 at 5% while oxygen remains at

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LOW OXYGEN TECHNICAL BULLETIN Models: IncuSafe Multigas Incubators | MCO-170M Physiological Normoxia, Physoxia, or Physiological Oxygen (approximately 3-7% O2) Most tissues do not experience oxygen levels at 20-21%. In our lungs, oxygen levels are around 14.5% and in peripheral tissues oxygen can be as low as 3.4-6.8%m. Thus, a more accurate representation of physiological oxygen levels tends to be between 3-7%, or most commonly 5% (Table 1). The term physiological normoxia (or just physoxia) is used to define oxygen levels between 3-7%, the same as the normal oxygen levels seen physiologically...

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LOW OXYGEN TECHNICAL BULLETIN Models: IncuSafe Multigas Incubators | MCO-170M Hypoxia (Pathological hypoxia: ~0.1% to 4.2% O2) Pathological hypoxia exists when the mechanisms to reverse oxygen depletion fail or become insufficient. Pathological hypoxia may occur in certain instances of loss or occlusion of blood vessels, or, in such cases as cancer, leaky and inadequate vasculature (Table 1) [1]. In these examples, oxygen levels tend to fall below 2%, but can range from 0.3-4.2% [1]. In many tumors, the medium tumor oxygen levels tend to be less than 2% [1]. Cancer research highly relies on maintaining...

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LOW OXYGEN TECHNICAL BULLETIN Models: IncuSafe Multigas Incubators | MCO-170M Hypoxia Incubator Chambers There are many types of hypoxic chambers. A hypoxic incubator chamber is a common tool used by scientists seeking to study cells at a lower oxygen level from 0.1-2%, but most offer the capability to adjust oxygen from 0.120%. These are most often used for pathological hypoxia studies. Multi-Plate Hypoxia Chamber used within a CO2 Incubator Reach-In “Glove Box” Hypoxia Work Station for Cell Handling Between Incubation Sessions Anaerobic Chamber Anaerobic chambers are used for applications, which...

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