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Simoa HD 1 Analyzer - 4 Pages

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Catalogue excerpts

Simoa HD-1 Analyzer The Simoa HD-1 Analyzer™ is a fully automated instrument for running immunoassays using Quanterix’ proprietary single molecule array, or Simoa, platform. Simoa is a digital form of ELISA, trapping and sealing individual immunocomplexes on paramagnetic beads in thousands of femtoliter sized wells in arrays found on the Simoa Discs. This isolation of each bead-immunocomplex allows the enzyme bound to the capture antibody to produce sufficient fluorescence in each well to be detectable, even when just a single molecule is present. As a result, the analyzer produces a map of the array - each of which contains more than 200,000 wells - where each well can be digitally analyzed as either “on” (containing a target molecule) or “off”. The proprietary Simoa algorithm then converts the measurement into a concentration which reaches femtomolar levels for most analytes. This revolutionary approach to digitalizing ELISA offers on average a 1000-fold improvement in sensitivity over current immunoassay techniques. In fact, Simoa has demonstrated sensitivity equivalent to PCR at a fraction of the cost and greater ease of use.

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Instrument Features and Benefits • Sensitivity: With an average of 1000x greater sensitivity than current immunoassays, analyte concentrations can be measured where they previously were not possible. Such a dramatic improvement in sensitivity opens new avenues of research and allows measurements of previously undetectable markers. Users with very low sample volumes can also pre-dilute samples to preserve samples and reduce matrix effects and still achieve greater sensitivity than ever before. Running immunoassays is a time consuming, largely manual technique. Users must either dedicate significant...

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Instrument Performance Metric < 10 pl requires pre-dilution step Measurements from Simoa™ (blue) of PSA in radical prostatectomy patients are well below the LOD (green line) of leading PSA diagnostic assay. Red measurements represent healthy controls. ELISA Kits Quanterix offers a broad range of complete ELISA kits as well as a robust homebrew list. Please visit www.quanterix.com for an updated list of all available assays and for detailed specifications. Contact sales@quanterix.com for assays available in prototype format and custom assay development services.

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Instrument Operation: The Simoa HD-1 Analyzer allows users to run either pre-packaged reagent kits from Quanterix or to develop their own assays using a robust homebrew protocol. The instrument was designed to automate the entire process, including making dilutions; mixing, washing, incubation steps, and finally read-out/analysis of the results. All system buffers, reagents, and waste are monitored on-board and users are alerted whenever action is required. About Quanterix Quanterix is a venture-capital backed company located in Lexington, MA. Founded in 2007 by Dr. David Walt, founder of Illumina,...

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