Celsis AMPiScreen® reagent
1 / 3ページ

Celsis AMPiScreen® Pharma Reagents for Rapid Microbial Detection Celsis AMPiScreen® Pharma reagents Adenosine triphosphate (ATP) bioluminescence provides an effective approach to microbial testing but is solely dependent • Results up to 75% faster than traditional methods can rapidly detect the presence of ultra-low levels of microbiological contamination up to 50% faster than traditional • Confirm the absence of microbial contamination for: -- Raw materials confident “Yes/No” answer, providing critical information whether to release or hold a product. upon microbial growth to achieve sufficient levels of ATP for detection. The Celsis AMPiScreen® Pharma reagent line ATP bioluminescence. The inherent subjectivity of manual enumeration or visual growth confirmation is removed with a The Celsis AMPiScreen® amplified-ATP assay is not constrained by the limited amount of metabolic ATP in the organism. -- In-process bioburden Instead, it produces additional ATP by using an enzyme-catalyzed reaction and an additional instrument-controlled -- Microbial limits amplification period to optimize the detection of microorganisms. -- Final sterility testing • Flexible kit configuration • Simplified reagent preparation • Compatible on both the Celsis Accel® and Celsis Advance II™ Based upon a proven detection method, Celsis AMPiScreen® products amplify low levels of contaminant ATP present in a test sample that are then easily detected by bioluminescence within Celsis® instruments. Reaction Principles The fundamentals are simple, yet powerful. Celsis® rapid microbial detection technology is based upon a naturally occurring, ATP-bioluminescence reaction, which also provides the characteristic glow of fireflies. Known as the luciferin-luciferase reaction, it occurs when the luciferase enzyme converts luciferin into oxyluciferin to produce light. Only when ATP is present will this reaction occur. Since ATP is present in all living cells, including prokaryotes, it can be used as a marker for contamination if a sample should not, under normal conditions, contain ATP .

The standard ATP bioluminescence detection principle, used in Celsis LumiScreen™ reagents, reproduces the luciferinluciferase reaction using reagents, where the presence of ATP molecules, derived from microbial contamination, generate light that is detected by a Celsis® luminometer. If no microbial contamination is present, the reaction does not occur and no light is produced. Traditional methods rely heavily on lengthy incubation periods to achieve visible growth, for colonies to grow large enough for confident enumeration in microbial limits, or for unambiguous turbidity in sterility...

Cross-Reagent Compatibility A single instrument can run both Celsis AMPiScreen® and Celsis LumiScreen™ reagents in back-to-back runs. The instrument’s reagents can be easily swapped followed by a simple rinse, wash, and prime process, all guided by on-screen prompts. Specific protocols can be pre-defined and saved using Celsis® instrument software for varying product types and reagents. Storage Conditions Reagent kits are stored at 2-8 °C when not in use. Once reconstituted, the stability of the reagents is 2 working days (up to 8 hours per day) at room temperature between 18-23 °C. Unused...