DMi8 S Platform
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Cutting of actin stress filaments labeled with mEOS in COS-7 cells. Courtesy: Dr. Ralf Jacob, Philipps University of Marburg, Marburg, Germany Illuminate. Irradiate. Obliterate. Pulsed Laser Unit – DMi8 S Whether you need to gently irradiate samples for DNA damage and uncaging, increase power levels for sub-cellular organelle perturbation and disruption, or cut and ablate through whole tissues, the Pulsed Laser Unit offers an all-in-one solution. Add this UV laser unit to the Infinity Scanner for precise targeting within the focal plane of your sample. The DMi8 S system also enables users to combine the Pulsed Laser Unit (PLU) with fiber coupled laser sources for a flexible, upgradable solution offering optogenetics, photobleaching and photoactivating on one universal system. • • • Scalpel to sledgehammer – all-in-one solution for gentle irradiation to whole tissue ablation Fully integrated into DMi8 S – Intelligent automation eliminates risk of damaging optical components. Customizable – record and cut in real-time or combine with multi-dimensional imaging Typical Applications: • Cutting • Ablation • DNA damage • Uncaging

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DMi8 S – Pulsed Laser Unit (PLU) Copyright © by Leica Microsystems CMS GmbH, Wetzlar, Germany, 2018 · Subject to modifications · LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. + Control: Timelapse imaging of cutting of a cell connection between COS-7 cells. Courtesy: Dr. Ralph Jacob, Philipps University of Marburg, Marburg, Germany 355 nm pulsed laser Pulse energy >15 µJ Peak power >13 kW Pulse Rep rate 2.5 kHz Pulse Width <1.1 ns Direct coupling to Infinity Scanner FS Laser safety Class 1 Power: Single pulse ablation of filamentous algae. Courtesy: Dr....

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