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Leica HCS A Amplify the Power of Imaging High Content Screening Automation

• Amplify the power of confocal imaging with Leica HCS A • Maximum ﬂexibility for universal applications • Easy-to-use automation provides efﬁcient high content screening • Powerful hardware for high resolution imaging and maximum content 2 Leica Design by Christophe

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High Content Screening (HCS) allows researchers to quickly change from descriptive to quantitative ﬂuorescence imaging during an experiment. High resolution imaging automation therefore answers complex questions in shorter time. It simpliﬁes research work and efﬁciently reveals relationships within and between cells and organisms. Leica Microsystems offers a set of innovative tools to convert your high resolution confocal microscope into a high content imaging device. Leica HCS A High Content Screening Automation Leica Microsystems provides a wide range of true spectral confocal imaging...

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Features • High resolution imaging • Time saving automation • Open architecture • Platform independent results • OME data formats • Perfect integration Modern research is a continuous cycle of experiment design, setup imaging, data handling, and analysis to discover live's processes and answer questions. Power of Imaging Result Content Analysis Data Processing Sample Preparation Automated Leica High Content Screening High resolution imaging techniques answer many questions in modern life science. For high content screening, automation is essential to efficiently achieve results. Intelligent...

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Sample eparation Leica High Content Screening Automation Data Management Content Analysis Research Result a Rat brain slice, small neuron network layer 5. Interneurons (Alexa 594, red) and Pyramidal Cell Oregon (Bapta 1, calcium sensitive, green). Courtesy of Dr. Thomas Nevian, Institute of Physiology, University of Bern, Switzerland. b Danio rerio – Zebraﬁsh – Nuclear and Acetylated α-Tubulin staining of 6 days ﬂh:eGFP Zebraﬁsh larvae Nuclei (Hoechst, blue), acetylated tubulin (red) and neurons (GFP, green). Courtesy of ICI Imaging Centre IGBMC, Illkirch, France. Choose the adequate...

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Many companies offer dedicated imaging routines for dedicated assays only. Leica Microsystems provides standard solutions for routine experiments plus maximum flexibility to freely adapt imaging. Features • Smart user interfaces • Workflow oriented wizards • Predefined templates • Easy adjustment • Quick start Easy-to-use AutomationWe Keep It Simple! Wizards guide the user through an experiment in a streamlined way. Design follows function - benefit from clear user interfaces, ensuring fast training and the highest productivity. Predefined Scanning Templates Place the specimen carrier on...

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Mouse diaphragm muscle stained against neuro-ﬁlament 150. Mosaic: xyz: 5 x 5 x 101 images Green: Alexa Fluor 488-secondary antibody and acetylcholine receptors (Red: Alexa Fluor 647-alpha-bungarotoxin). Courtesy of Dr. R. Rudolf, Cellular Signaling in Skeletal Muscle, Karlsruhe Institute of Technology, Germany.

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Automated High Content Screening Simplifies Da LAS AF software simpliﬁes routines. Leica Microsystems’ goal is to make daily work as easy as possible so researchers can concentrate on the results, not on the imaging process. We Keep it Simple! LAS AF MATRIX Mosaic Fine details as well as an overview are important when evaluating experimental results. Today, simple routine tasks, such as stitching of individual images are challenging. Leica HCS A provides entirely new designed mosaic algorithms for excellent results at the push of a button. Leica LAS AF MATRIX Mosaic generates large high...

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Daily Routines High Resolution Single Image High Content Mosaic Fast Multiwell Plate Screening High Content Information

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Gain Flexibility Flexible scanning conditions, even on the smallest scan ﬁeld level, match a full variety of experiments. Adjustable Scanning Templates No need to be a programmer – new scanning templates can be designed at micron scale to easily ﬁt chamber slides, multiwell plates or simple spotter arrays. Once deﬁned, the templates are ready to use for all applications and can even be conveniently shared between laboratories or communities. Imaging without Limits – MultiJob and MultiPositioning Feel free to combine a variety of individual scan jobs for any area of interest of the specimen....

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Autofocus Routines Five autofocus algorithms are available, optimized for different setups. The suitable routine is selected from a pull-down menu. After the initial scan, the software automatically creates a focus map with true sample topology. This map is used for fast, accurate z-positioning during the scan. According to the size and planarity of the samples, the optimal number and positions of the autofocus points can be freely deﬁned. Z-Drift Compensation Live specimens can grow in long-time measurements, changing the z-position of interest. Microscope conditions can change due to...

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Automated High Content Screening for Quant Detect Rare Events On-the-Fly! LAS AF MATRIX Developer Suite Highly complex assay designs are now possible with LAS AF MATRIX Developer Suite. The Mitocheck project (1), conducted at the EMBL in Heidelberg, is an excellent example of comprehensive and ﬂexible automation: The self-acting process automates mitosis identiﬁcation. High Content Screening with Fast Pre-Scan Pre-Scan – Object Identiﬁcation At ﬁrst, a fast, low resolution pre-scan of sample plates is routinely performed to identify the events of interest (1). After each scan, OME-image...

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h Interactive System Control: Fully Automated Mitosis Acquisition High Content Scan Feedback Automation Image Analysis embl ijjjjjjjl 1. Pro-Scan 2. Analysis 3. Classification Segmentation Feature Extractor Object Object Selection

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The Perfect MatchData Interfaces Open architecture for truly platform independent information exchange in an interactive environment - this is the goal attained by the new Leica HCS A data model. Experiment Meta Data Administration Experiment IDs, description, and meta data can be entered manually or by barcode. Additional experiment information can be added to the existing XML meta data file by external programming to provide comprehensive result data sets. Platform Independent Results Distribution Leica HCS A imaging formats can be used platform independent on Apple MAC™ OS, Microsoft...

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HCS A
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