Nanodigmbio-NadPrep EZ DNA Library Preparation Kit v2
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NadPrep EZ DNA Library Preparation Module v2Introduction NadPrep EZ DNA Library Preparation Kit v2 is designed for the preparation of high-quality sequencing libraries from double stranded DNA (dsDNA) on Illumina and MGI platforms. To simplify the experimental process, multiple processes were combined into one single step, including the fragmentation, end repair and adapter ligation. This A-T ligation-based kit can be used for whole genome sequencing with DNA input ranging from 5 to 500 ng, and is compatible with hybridization capture based targeted sequencing. • Suitable for various types of samples including gDNA, FFPE B+/B/C/D DNA samples • The length of DNA fragment is flexible and controllable • Flexible system and simple operation, with Fragmentation, End Repair and A-tailing being completed in one step • Generate high quality libraries with uniform and unbiased coverage as well as low GC bias • Low background noise (abnormal sequence introduced by enzymatic digestion by-products) • Compatible with automatic workstations MGI gDNA/FFPE DNA Duration Illumina Fragmentation, End Repair 40-60 min and A-tailing

Product Performance High Efficiency and Stability Input gDNA Input gDNA Fig 1. Library yield of NadPrep EZ DNA Library Preparation Kit v2 for gDNA samples with different input amounts. The libraries were prepared by using NadPrep EZ DNA Library Preparation Module v2 (NadPrep Enfrag v2) coupled with A. NadPrep Universal Stubby Adapter (UDI) Module and NadPrep UDI Adapter Module (for Illumina®), and B. NadPrep Universal Adapter (SI) Module (for MGI) and NadPrep Universal Adapter (MDI) Module (for MGI) respectively, with 25 min of enzymatic digestion and recommended PCR cycles. Compatible with...

Fig 3. Size distribution of libraries prepared with different types of samples under different enzymatic digestion conditions. A. The libraries were prepared from 250 ng of gDNA sample with different grades by using NadPrep EZ DNA Library Preparation Module v2 coupled with NadPrep UDI Adapter Module (for Illumina®), with enzymatic digestion at different times as protocol, so as to obtain DNA fragments of different lengths. B. The libraries were prepared from 50 ng of FFPE DNA samples of different grades, with enzymatic digestion at different times as protocol, so as to obtain DNA fragments...

Low Background Noise Introduced Library yield Artifact rate Artifact rate (%) Artifact rate (%) Fig 5. The abnormal reads ratio of libraries prepared with different types of samples by using different library preparation kits. The libraries were prepared with gDNA and different grades of FFPE DNA samples (50 ng each) with library preparation strategies, including NadPrep Sonication, NadPrep Enfrag v2, Vendor Q *, Vendor K* and Vendor V* enzymatic fragmentation library preparation kits. The multi-hybridization reaction was initiated with 500 ng of each library with the same library...

Ordering Information Type NadPrep EZ DNA Library Preparation Module v2, 96 rxn NadPrep EZ DNA Library Preparation Module v2, 24 rxn Library Prep Module NadPrep Universal Adapter (SI) Module Set A1 (for MGI), 24 rxn NadPrep Universal Adapter (SI) Module Set B1 (for MGI), 96 rxn NadPrep Universal Adapter (MDI) Module Set D1 (for MGI), 480 rxn NadPrep Universal Adapter (MDI) Module Set D3 (for MGI), 480 rxn NadPrep Universal Adapter (MDI) Module Set B3 (for MGI), 96 rxn NadPrep Universal Adapter (MDI) Module Set B4 (for MGI), 96 rxn NadPrep Universal Adapter (MDI) Module Set D2 (for MGI), 480...