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DIC Microscopy

DIC Microscopy
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Differential Interference Contrast (DIC) microscopy is a contrast technique that allows transparent structures to be visualized by exploiting changes in refractive index. Unstained biological specimens usually do not alter the amplitude of the incident light, i.e., they are phase specimens. Usually, detectors (eye, camera etc.) are sensitive only to the intensity. Hence, visualization of phase specimen or reliefs requires some special method to convert phase information to intensity information, with the high contrast and 3-D relief effect that is characteristic of DIC.

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DIC MAIN APPLICATIONS DIC microscopy is ideal for unstained living specimens (like cultured cells, embryos, blood smears, diatoms, protozoa) and provides morphological information without the need to use potentially toxic dyes and fluorophores. DIC is, however, often used in association with fluorescence to reveal morphological features of the specimen. In metallography applications, images created in reflected light DIC can be inter reted as a true three-dimensional representation of p the surface geometry: a clear distinction can be realized between raised and lowered regions in the...

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Köhler DIC (Differential Interference Contrast) on transmitted light B-1000BF M-1198 DIC slider with Nomarski prism Illumination: OPTIKA X-LED8 illumination, on all B-1000. DIC Set: OPTIKA M-1198 - Köhler DIC for transmitted light (including DIC slider with Nomarski prism, polarizing and IF550 filters) *. Objectives: Semi-APO IOS U-PLAN F M-1075 - 4x/0.13 M-1076 - 20x/0.50 M-1077 - 10x/0.30

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Illumination: 100 W halogen (IM-5MET & B-1000MET) or 18 W LED (B-1000MET only) illumination. DIC Slider: OPTIKA M-870 - DIC slider with Nomarski prism for reflected light. v 1.0 2018 - OPTIKA reserves the right to make corrections, modifications, enhancements, improvements and other changes to its products at any time without notice. Headquarters and Manufacturing Facilities OPTIKA® S.r.l. Via Rigla, 30 - 24010 Ponteranica (BG) - ITALIA - Tel.: +39 035.571.392 - Fax: +39 035.571.435 - info@optikamicroscopes.com Optika Sales branches OPTIKA® Spain spain@optikamicroscopes.com OPTIKA®...

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