1300 Series B2 Class II, Type B2 Biological Safety Cabinet
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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 1

1300 Series B2 Class II, Type B2 Biological Safety Cabinet Operating Instructions 3848304 Rev. G

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 2

Copyright These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or digital postprocessing or reproduction, even in part, are only allowed with the written consent of the Thermo Fisher Scientific Inc. This regulation does not apply to reproductions for in-plant use. Trademarks All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers. Thermo Fisher Scientific Inc Controlled Environment Equipment 401 Millcreek Road, P.O. Box 649, Marietta, Ohio 45750-0649 United States

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 3

Operating Instructions Safety Cabinet | 1300 Series B2

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 4

Operating Instructions Safety Cabinet | 1300 Series B2 Figures Figure 2-1 Figure 3-1 Figure 3-2 Figure 3-3 Figure 3-4 Figure 4-1 Figure 4-2 Figure 4-3 Figure 4-4 Figure 5-1 Figure 5-2 Figure 5-3 Figure 5-4 Figure 6-1 Figure 6-2 Figure 6-3 Figure 6-4 Figure A-1 Figure B-1

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 5

Operating Instructions Safety Cabinet | 1300 Series B2 Introduction Dear User, Congratulations on your purchase of a Thermo Scientific 1300 Series B2 Class II, Type B2 biosafety cabinet. Your 1300 Series B2 biosafety cabinet has been tested and certified in accordance to NSF/ANSI 49, and is designed to protect the user, the environment, and your research from harmful substances and cross-contamination. This user’s manual provides instructions on how to use the 1300 Series B2 most effectively and safely. The 1300 Series B2 offers a unique range of product features that will enhance your...

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Operating Instructions Safety Cabinet | 1300 Series B2 Introduction Instruction of the Operating Personnel The operating instructions describe the safety cabinet 1300 Series B2, and applies to the models: • • This safety cabinet has been manufactured in keeping with the latest technological developments and has been tested before delivery for its correct function. It may, however, present potential hazards if it is not used according to the intended purpose or outside of operating parameters. Therefore, the following procedures must always be observed to prevent accidents: • • The safety...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 7

Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Before you install the Biosafety Cabinet, you need to prepare the site for installation. Carefully examine the location where you intend to install the cabinet. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power must be located near the installation site. Carefully read this chapter to learn: • Location requirements. Electrical power requirements. Exhaust requirements. Service utility requirements. Space requirements. Refer to Appendix C:...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 8

Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Suggested Location Note: The Biosafety Cabinet should be located away from traffic patterns, doors, fans, ventilation registers, fume hoods and any other air-handling devices that could disrupt its airflow patterns. All windows in the room should remain closed. Figure 2-1 shows the preferred location for the Biosafety Cabinet. Preferred location Air register blocked or redirected to prevent cabinet disruption Alternate location

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 9

Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Exhaust Requirements Before deciding on a location, examine it carefully to ensure that it is compatible with the cabinet’s exhaust duct. The area directly above the cabinet’s exhaust port should be clear of structural elements, water and utility lines, or other fixed obstructions. There should be enough clearance to allow for the passage of a 10" PVC or stainless steel duct. Avoid cabinet locations that require either an elbow directly on top of the cabinet’s exhaust connection or an excessive number of elbows to clear...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 10

Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Service Line Requirements All utility service lines should be ¼ inch O.D., brass, copper, or stainless steel, and equipped with an easily accessible shut-off valve. If the service line pressure exceeds 100 PSI, it must be equipped with a pressure regulator to reduce the line pressure. Consult local regulations for materials, pressures and techniques regarding gas services. Note: The use of flammable gases or solvents should be avoided in the Biosafety Cabinet. Open flame in the cabinet will disrupt the laminar airflow in...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 11

Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Now that the installation is properly prepared, you are ready to inspect, install, and certify the Biosafety Cabinet. This chapter covers how to: • Unpack and move the Biosafety Cabinet. Connect the electrical supply source. Connect the service lines. Connect to an exhaust system. Arrange certification of the Biosafety Cabinet. Tools required for installation the Biosafety Cabinet include two 1/2" wrenches, a flat-blade screwdriver, a #2 Phillips screwdriver, and a carpenter’s level. Note: The Biosafety Cabinet models...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 12

Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Unpacking the Biosafety Cabinet Carefully remove the outer carton and inspect the cabinet for damage that may have occurred in transit. If the Biosafety Cabinet is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier. Note: United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery. Do not return goods without the prior authorization of Thermo Fisher Scientific. Unauthorized...

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1300 Series B2 Class II, Type B2 Biological Safety Cabinet - 13

Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Installing the Biosafety Cabinet on a Base Stand Thermo Scientific offers accessory Base Stands that effectively support the weight of the cabinet. Stands for a B2 cabinet generally are not mobile, since it is connected to an exhaust duct. Telescoping Base Stands Adjust the leg height before installing the cabinet on top of the stand. The height can be set in 1-inch intervals between 27.5 to 34.5 inches (699 to 876 mm), providing a Cabinet work surface height from 30.0 to 37.0 inches (762 to 940 mm). The Base Stands for...

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