The Power of Massively Parallel Partitioning Inside the Chromium Controller

Chromium | The Power of Massively Parallel Partitioning The Power of Partitioning The Chromium System, powered by GemCode Technology, provides a precisely engineered reagent delivery method that enables thousands of micro-reactions in parallel. Single T cell Single Cell GEMs Massive Partitioning and Barcoding Encapsulate your sample into hundreds to tens of thousands of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream analysis. Each Gel Bead, infused with millions of unique oligonucleotide sequences, is mixed with a sample, which can be high molecular...

Gel Bead TruSeq Read 1 Nextera Read 1 (Read 1N) Single Cell 3’ v3 Gel Bead Nextera Read 1 (Read 1N) Single Cell Partitioning for Transcriptome Analysis Cells Enzyme Collect The Chromium System also enables single cell transcriptional profiling of up to tens of thousands of single cells. Single cell suspensions loaded onto the system are partitioned into GEMs, where transcripts are tagged with cell-specific barcodes. The barcoded cDNA is then pooled for downstream processing and library preparation (Figure 2). Our precise and efficient microfluidics allow 100–80,000+ cells to be recovered in droplets...

Chromium | The Power of Massively Parallel Partitioning Switch Oligo Single Cell Partitioning for Immunology Applications Collect Immune profiling of up to tens of thousands of T and B cells on a cell-by-cell basis can also be performed with the Chromium System. Single cell suspensions, including flow sorted cells, or cells labeled with antibodies or peptide-MHC multimers using Feature Barcoding technology, are loaded into the system and partitioned into GEMs. Transcripts are generated and tagged with cell-specific barcodes and the cDNA is then pooled for downstream processing and library Single...

Single Nuclei Partitioning for Epigenome Analysis Transposition of Nuclei in bulk Oil The Chromium System enables the analysis of chromatin accessibility at single cell resolution. In the Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin) workflow, single nuclei are first treated in bulk with transposase enzyme to preferentially insert sequencing adaptors into accessible DNA regions. Transposed nuclei are then partitioned into GEMs in the Chromium Controller. All accessible DNA fragments from the same nucleus share a common 10x barcode. Single Nucleus GEMs The barcoded, accessible...

Chromium | The Power of Massively Parallel Partitioning Partition Step #1 Cell Beads Cell Lysis Single Cell Partitioning for Genome Analysis Using the 10x technology, DNA from single cells is prepared in a twostep partitioning process. In the Chromium Single Cell DNA1 workflow, single cells are first encapsulated in hydrogel Cell Beads (CBs), allowing the cells to be subjected to protein digestion and denaturation, while still retaining intact DNA in the hydrogel matrix (Figure 5). After the cells have been lysed, CBs containing DNA are partitioned with 10x barcoded Gel Beads (GBs), to generate...

High Molecular Weight DNA Partitioning For whole genome2 or exome2 analyses, the Chromium Controller allows researchers to create sequencing-ready libraries with >1,000,000 unique barcodes from ~1 ng of HMW genomic DNA (Figure 6). Massive partitioning of the genome provides haplotype level dilution and enables the barcoding of long input DNA molecules, which are then sequenced in bulk to produce a unique data-type known as Linked-Reads. The long range information encoded in barcoded Linked-Reads is leveraged by innovative bioinformatics pipelines to assemble sequences over long genomic distances,...

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