Inside Chromium Next GEM Technology
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Inside Chromium Next GEM Technology

How it works: From cells and nuclei to sequencing-ready libraries Resolving biology with scalable, single cell multiomic solutions Gel Beads, cells or nuclei, enzymes, and partitioning oil are loaded onto a Next GEM chip. Within the Chromium instrument, barcoded Gel Beads are mixed with the cells or nuclei, enzymes, and partitioning oil to form tens of thousands of GEMs. The Chromium platform, powered by Next GEM technology, enables integrated analysis of single cells at massive scale. Our suite of Chromium Single Cell products can capture molecular readouts of cell activity in multiple...

Chromium assays Chromium Single Cell Gene Expression Chromium Single Cell Immune Profiling Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to explore cellular heterogeneity, discover novel targets, and identify biomarkers with combined detection of surface protein expression or CRISPR edits in each cell. While Next GEM technology enables single cell analysis, Feature Barcode sequences expand the breadth of analytes that can be captured at single cell resolution. Chromium Single Cell Immune Profiling provides a multiomic...

Targeted Gene Expression Profile a defined set of transcripts to reduce sequencing costs while increasing sample number or sequencing depth with customizable, comprehensive gene panels. Compatible assays: Chromium Single Cell Gene Expression, Chromium Single Cell Immune Profiling Sample input Whole transcriptome barcoded library Starting with a final, barcoded library, up to eight input libraries can be pooled before target enrichment using hybrid capture. Enriched libraries can be sequenced at up to 90% lower cost than that of a corresponding whole transcriptome library. Target enrichment...

Chromium instrument family Chromium instruments use advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes, enabling a seamless workflow from single cell or nuclei suspensions to sequencer-ready libraries. Chromium X Series Chromium Controller Chromium Connect Instrument dimensions Instrument weight Supported workflow Supported single cell assays All assays and throughputs (low, standard, and high) All assays; standard and low throughputs only Gene Expression Immune Profiling Contact us 10xgenomics.com | info@10xgenomics.com © 2021 10x Genomics, Inc....