Inside Chromium Next GEM Technology

Product catalog summary

Overview of Chromium Next GEM Technology: The Chromium platform, powered by Next GEM technology, facilitates large-scale single-cell analysis. It captures molecular readouts across various dimensions such as gene expression, chromatin accessibility, and immune profiling. The technology's core is its ability to generate numerous single-cell partitions, each with a unique barcode for analysis.

Core Technology: The platform uses Gel Beads coated with unique oligonucleotide barcode sequences. These beads are encapsulated with single cells or nuclei in a micro-reaction environment, allowing for the capture and barcoding of molecules of interest. This process results in sequencing-ready libraries.

Feature Barcode Technology: This technology expands the range of analytes captured at single-cell resolution. It uses oligonucleotide barcode sequences to label antibodies or CRISPR guides, enabling simultaneous measurement of gene expression and additional cellular features.

Chromium Single Cell Assays: The platform supports various assays, including:

Gene Expression: Captures 3' mRNA and other features like surface protein expression or CRISPR edits.
Immune Profiling: Analyzes B-cell or T-cell receptors, surface protein expression, and antigen specificity.
ATAC: Analyzes chromatin accessibility to understand gene regulatory mechanisms.
Multiome ATAC + Gene Expression: Profiles gene expression and open chromatin from the same cell.

Sample Multiplexing and Targeted Gene Expression: The platform allows for sample multiplexing using 3' CellPlex, enabling the combination of up to 12 samples. Targeted gene expression reduces sequencing costs by focusing on specific transcripts.

Chromium Instrument Family: The instruments use advanced microfluidics for single-cell partitioning and barcoding. They support various workflows and throughputs, with options for manual or automated processes.

Applications and Panels: The platform supports customizable gene panels for various research areas, including cancer, immunology, and neuroscience.

Catalog excerpts

Inside Chromium Next GEM Technology

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How it works: From cells and nuclei to sequencing-ready libraries Resolving biology with scalable, single cell multiomic solutions Gel Beads, cells or nuclei, enzymes, and partitioning oil are loaded onto a Next GEM chip. Within the Chromium instrument, barcoded Gel Beads are mixed with the cells or nuclei, enzymes, and partitioning oil to form tens of thousands of GEMs. The Chromium platform, powered by Next GEM technology, enables integrated analysis of single cells at massive scale. Our suite of Chromium Single Cell products can capture molecular readouts of cell activity in multiple dimensions,...

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Chromium assays Chromium Single Cell Gene Expression Chromium Single Cell Immune Profiling Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to explore cellular heterogeneity, discover novel targets, and identify biomarkers with combined detection of surface protein expression or CRISPR edits in each cell. While Next GEM technology enables single cell analysis, Feature Barcode sequences expand the breadth of analytes that can be captured at single cell resolution. Chromium Single Cell Immune Profiling provides a multiomic solution...

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Targeted Gene Expression Profile a defined set of transcripts to reduce sequencing costs while increasing sample number or sequencing depth with customizable, comprehensive gene panels. Compatible assays: Chromium Single Cell Gene Expression, Chromium Single Cell Immune Profiling Sample input Whole transcriptome barcoded library Starting with a final, barcoded library, up to eight input libraries can be pooled before target enrichment using hybrid capture. Enriched libraries can be sequenced at up to 90% lower cost than that of a corresponding whole transcriptome library. Target enrichment via...

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Chromium instrument family Chromium instruments use advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes, enabling a seamless workflow from single cell or nuclei suspensions to sequencer-ready libraries. Chromium X Series Chromium Controller Chromium Connect Instrument dimensions Instrument weight Supported workflow Supported single cell assays All assays and throughputs (low, standard, and high) All assays; standard and low throughputs only Gene Expression Immune Profiling Contact us 10xgenomics.com | [email protected] © 2021 10x Genomics, Inc. FOR...

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