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AMPLIQUALITY HPV-TYPE EXPRESS v3.0 Fast system for detection and genotyping of Human Papillomavirus by Single-Step PCR and Reverse Line Blot The system can identify the following 40 HPV genotypes: 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68 (a e b), 69, 70, 71, 72, 73, 81, 82, 83, 84, 87, 89, 90. All genotypes, including not listed strains, are detected by the Universal HPV Sequence. The test has been validated for cytological samples (cervical, urethral, oral swabs) and histological samples (fresh, frozen, formalin-fixed and paraffin-embedded biopsy tissue). The co-amplification of a housekeeping gene, allows the user to test the quality of the DNA extracted; this internal control is detected on the strip by a dedicated band. The PCR step employs the dUTP/UNG system in order to prevent carry-over contamination. AMPLIQUALITY HPV-TYPE EXPRESS v3.0 has passed the WHO proficiency test. Cervical cancer is the second most frequent cancer in women in the world (WHO 2008). A persistent infection by at least one of several strains of high-risk human papillomaviruses (HR-HPV) is the initial step in the development of many cervical carcinoma [1-2]. While HPV infections are frequent in sexually active individuals, only a persistent infection gives rise to precancerous cervix lesions and eventually cancer. Such a persistent HPV-infection does only occur in a small portion of cases and it is always connected to high-risk HPV genotypes. AMPLIQUALITY HPV-TYPE EXPRESS v3.0 is a fast and reliable system for HPV detection and genotyping by Single-Step PCR and Reverse Line Blot.

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SAMPLE PRE - TREATMENT AND DNA EXTRACTION PRODuCT DESCRIPTION: • Ready-to-use complete PCR MASTERMIX: you only need to add sample DNA to an aliquot of the mastermix • INTERNAL CONTROL: coamplification of a housekeeping gene • POSITIVE CONTROL: plasmid DNA containing a fragment of the HPV 61 genome • Strips and reagents for visualization of the HPV genotypes: analysis of PCR products by Reverse Line Blot • Optional automation of strip development by using the AutoBlot 3000H (MedTec) or ProfiblotTM T48 (Tecan); the automation minimizes hands-on time and increases accuracy • CE ivD marked...

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