AccuPure Circulating DNA

Product catalog summary

Specification
The AccuPure Circulating DNA Mini Kit (D11096) is designed for automated extraction on the iColumn 12/24 System. It is suitable for serum, plasma, or cell-free body fluids with a sample volume of 800 μl and an elution volume of 50 μl. The kit can recover cfDNA ≥ 50 bp, with typical DNA recovery ranging from 1-100 ng/ml of plasma or serum. The method is column-based, specifically for the iColumn 12/24 System, and is applicable for cancer diagnosis, monitoring cancer therapy response, non-invasive prenatal testing (NIPT), and biomarker discovery.

Features
The kit is fully automated on the iColumn 12/24 System and uses a column-based method without the need for organic solvents. It allows for the concentration of cfDNA with high input and low elution volumes, effectively removing contaminants and inhibitors. The kit is designed to purify circulating tumor DNA and fetal DNA from cell-free samples, addressing the challenge of removing inhibitors from larger sample volumes while minimizing cfDNA loss. The Trinity Technology™ used in the iColumn system enhances yield and purity compared to magnetic bead methods.

Workflow
The workflow involves adding lysis buffer, carrier RNA, and PK to the sample, followed by processing on the iColumn 12/24 System.

Ordering Information
The kit is available as AccuPure Circulating DNA Mini Kit (96 reactions) with Cat. No. D11096. The iColumn 12 and 24 Automated DNA/RNA Purification Systems are available with Cat. Nos. ABM1012 and ABM1024, respectively.

Performance
The kit provides superior yields compared to bead systems, with purified cfDNA quantified by the Qubit system. Concentrations lower than 0.5 ng/ml are not detectable. PCR results show a higher success rate in amplification and sequencing with the iColumn system. The system effectively removes contaminants and inhibitors for PCR or qPCR. Comparative data between iColumn and bead systems demonstrate higher cfDNA yield and successful KRAS mutation detection with the iColumn system.

Catalog excerpts

AccuPure Circulating DNA Mini Kit For automated extraction on iColumn 12/24 System Purify cfDNA from serum, plasma or body fluid This kit is designed to purify circulating tumor DNA and circulating fetal DNA from cell free samples. In order to catch more cfDNA, it’s quite normal to enlarge the sample volume. But once sample volume increased, inhibitors also accumulated. So, the challenge of cfDNA purification is how to remove increased inhibitors come from larger sample volume effectively and lose the very rare cfDNA as few as possible. By iColumnTM, we utilize Trinity TechnologyTM to catch cfDNA by column instead of magnetic beads which not only get higher yield but also with higher purity. FEATURES • Fully automated on iColumn 12/24 System • Column based method • No organic solvents required • Concentration of cfDNA, high input and low elution volumes • Removal of contaminants and inhibitors WORKFLOW 800 μl Serum Plasma SPECIFICATION AccuPure Circulating DNA Mini Kit (D11096) Serum, Plasma or Cell free body fluids Sample type 800 μl Sample volume 50 μl Elution volume Able to catch cfDNA ≥ 50 bp, typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. (The yield DNA Recovery varies depending on the sample source and the health situation of the donor.) Column based Method For iColumn 12/24 System System 1.Cancer Diagnosis 2.Monitoring the Response to Cancer Therapy Applications 3.Non-Invasive Prenatal Testing (NIPT) 4.Biom

Open the catalog to page 1

PERFORMANCE • Superior yields than other beads systems No. 1 2 3 4 5 6 7 8 9 10 PC NTC Purification System iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads --- QPCR (GAPDH) *Purified cfDNA was quantified by Qubit system. Concentration lower than 0.5ng/ml is not detectable and showed as N/A. After one time of low speed centrifuge and another time of high speed centrifuge, 800 μl of plasma sample was collected and applied to iColumn system and Beads system in parallel to purify cfDNA. • Removal of contaminants...

Open the catalog to page 2

AccuPure Circulating DNA

AccuPure Circulating DNA

Catalog excerpts

All Accubiomed CO., Ltd catalogs and technical brochures