AccuPure Circulating DNA Mini Kit For automated extraction on iColumn 12/24 System Purify cfDNA from serum, plasma or body fluid This kit is designed to purify circulating tumor DNA and circulating fetal DNA from cell free samples. In order to catch more cfDNA, it’s quite normal to enlarge the sample volume. But once sample volume increased, inhibitors also accumulated. So, the challenge of cfDNA purification is how to remove increased inhibitors come from larger sample volume effectively and lose the very rare cfDNA as few as possible. By iColumnTM, we utilize Trinity TechnologyTM to catch cfDNA by column instead of magnetic beads which not only get higher yield but also with higher purity. FEATURES • Fully automated on iColumn 12/24 System • Column based method • No organic solvents required • Concentration of cfDNA, high input and low elution volumes • Removal of contaminants and inhibitors WORKFLOW 800 μl Serum Plasma SPECIFICATION AccuPure Circulating DNA Mini Kit (D11096) Serum, Plasma or Cell free body fluids Sample type 800 μl Sample volume 50 μl Elution volume Able to catch cfDNA ≥ 50 bp, typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. (The yield DNA Recovery varies depending on the sample source and the health situation of the donor.) Column based Method For iColumn 12/24 System System 1.Cancer Diagnosis 2.Monitoring the Response to Cancer Therapy Applications 3.Non-Invasive Prenatal Testing (NIPT) 4.Biom

PERFORMANCE • Superior yields than other beads systems No. 1 2 3 4 5 6 7 8 9 10 PC NTC Purification System iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads iColumn Beads --- QPCR (GAPDH) *Purified cfDNA was quantified by Qubit system. Concentration lower than 0.5ng/ml is not detectable and showed as N/A. After one time of low speed centrifuge and another time of high speed centrifuge, 800 μl of plasma sample was collected and applied to iColumn system and Beads system in parallel to purify cfDNA. • Removal of contaminants...