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AccuPure FFPE Tissue DNA

AccuPure FFPE Tissue DNA

AccuPure FFPE Tissue DNA

Product catalog summary
Specification
AccuPure FFPE Tissue DNA Mini Kit (D22096) is designed for automated extraction on the iColumn 12/24 System. It is suitable for 5-10 μm FFPE sliced sections, with an elution volume of 50 μl, using a column-based method. Applications include cancer diagnosis, biomarker searching, PCR, QPCR, and MASS.

Features
The kit provides NGS Grade DNA/RNA for wide applications and includes a non-organic de-waxing buffer. It significantly reduces hands-on time from 3 hours to 15 minutes and offers a fully automated process from deparaffination to elution. It can separate liquid lysate from tissue debris.

Performance
The kit ensures high yield and integrity, with DNA quality comparable to manual methods. It allows for high purity DNA extraction suitable for QPCR without inhibition. The iColumn system provides less fragment damage and maintains DNA size integrity.

Workflow
The process involves adding DWX Buffer, vortexing, adding Lysis Buffer, vortexing again, and adding PK with pipetting.

Ordering Information
AccuBioMed Co., Ltd. offers the AccuPure FFPE Tissue DNA Mini Kit (96 reactions) and the iColumn 12/24 Automated DNA/RNA Purification Systems with catalog numbers D22096, ABM1012, and ABM1024 respectively.

Conclusion
The AccuPure FFPE Tissue DNA Mini Kit provides an efficient and automated solution for extracting high-quality DNA from FFPE samples, facilitating various molecular diagnostic applications.
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Catalog excerpts

AccuPure FFPE Tissue DNA-1

AccuPure FFPE Tissue DNA Mini Kit For automated extraction on iColumn 12/24 System Extract the hidden treasure of your FFPE samples Billion of Formalin-Fixed, Paraffin-Embedded (FFPE) tissue samples are lie in hospitals and bio-banks world wide. To decipher genetic codes inside of these treasure-trove, the very first step is to extract nucleic acid of it. However, this preservation method of tissue is originally for pathological sections observed under microscope, instead of molecular diagnostic. Nucleic acids inside of FFPE samples are always fragmented and crosslinked with other substrate inside of cell. Therefore, it’s difficult to extract pure and intact nucleic for following applications. In addition, it’s a tedious and time consuming work from deparafinization, decrosslinking to lysis and extraction. Here we bring a fully automated extraction solution for your FFPE samples. FEATURES • NGS Grade DNA/RNA for wide application • Non-organic de-waxing Buffer • Reduce hands on time from 3 hr to 15 min • Fully automated from Deparaffination to Elution • Able to separate liquid lysate with tissue debris 5-10 μm FFPE sliced sections SPECIFICATION AccuPure FFPE Tissue DNA Mini Kit (D22096) 5-10 μm FFPE sliced sections (up to 5 sections) Sample type 50 μl Elution volume Column based Method For iColumn 12/24 System System 1.Cancer Diagnosis Applications 2.Biomarker searching 3.PCR, QPCR, MASS • High Yield- Quantity comparison by spectrophotometer 150 iColumn Manual

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AccuPure FFPE Tissue DNA-2

PERFORMANCE • High Integrity - Quality comparison by capillary electrophoresis FFPE tissue DNA can be purified by iColumn Purification System with less fragment damage. Purified DNA size of iColumn and manual purification were distributed on the same range in the figure. • High Purity - Purity Comparison by QPCR 40 Using manual and iColumn purification system could obtain pure DNA, which can be used immediately on QPCR without inhibition. ORDERING INFORMATION Product AccuPure FFPE Tissue DNA Mini Kit (96 rxn) iColumn 12 Automated DNA/RNA Purification System iColumn 24 Automated DNA/RNA Purification...

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