Data Sheet Genomic DNA Analysis Agilent Fragment Analyzer Systems Efficient assessment of genomic DNA size, quality and quantity Quality assessment of genomic DNA (gDNA) is essential in several downstream applications including cloning, restriction-digest screening, microarray, and nextgeneration sequencing. Completing gDNA quality assessment in a time efficient manner increases lab productivity and decreases time to results. The Agilent Fragment Analyzer systems streamline gDNA analysis. There are two kits designed specifically for this purpose. The Agilent Genomic DNA 50 kb kit analyzes high concentration samples from 25 to 250 ng/µL with a single 200-fold dilution while the Agilent HS Genomic DNA 50 kb kit is ideal for the analysis of low concentration samples from 0.3 to 12 ng/µL. Both kits use the Genomic Quality Number (GQN) metric, a user-defined threshold. Using this metric, each sample is assigned a GQN, allowing for efficient assessment of a sample by applying a score between 0 and 10 based on the amount of sample that exceeds the threshold. A higher GQN score indicates a higherquality sample. Features and benefits – Extended gDNA sizing range Accurately and precisely size gDNA samples from 75 bp through 60 kb. – Short run times Separate samples in 60 minutes (short capillary array) or less. – Genomic Quality Number (GQN) Determine genomic DNA integrity using a flexible threshold based on sample type and needs. Agilent Genomic DNA 50 kb kit, 500 samples Agilent HS Genomic DNA 50 kb kit, 500 samples

Figure 1. Analysis of four gDNA samples on a Fragment Analyzer system with the Genomic DNA 50 kb kit. Prosize data analysis software provides both (A) an electropherogram and (B) a digital gel image for visual quality analysis. The dotted blue line on the electropherogram indicates a user-defined threshold, in this example 10,000 bp. (C) Using this threshold, each sample is assigned a Genomic Quality Number (GQN), allowing for efficient assessment of a sample by applying a score between 0 and 10 based on the amount of the sample that exceeds the threshold. A higher GQN score indicates a higher-quality...