Data Sheet Large Fragment 50 kb Analysis Agilent Fragment Analyzer Systems Assessment of large DNA fragment size, quality, and quantity Quality assessment of large DNA fragments and smears is essential in various downstream applications such as long-read next-generation sequencing (NGS). The long-read sequencing workflow can have several quality assurance steps, including starting DNA material, sheared DNA pre-library formation, and the final long-read NGS library. The Agilent Fragment Analyzer systems are recommended for sizing and quantification, and greatly decreases the time and sample input required for routine quality control steps. With the Agilent HS Large Fragment 50 kb kit, users can achieve accurate and reliable quality assessment and sizing of DNA through 50 kb. Features and benefits – Simple and convenient sample handling Requires only 2 µL of sample for analysis, with a broad input concentration range of 5 to 600 pg/µL for DNA fragments or 50 pg/µL to 5 ng/µL for DNA smears. – Large sizing range Accurately size DNA fragments and smears from 75 bp to 50 kb. – Short run times Complete analysis in approximately one hour (using a short capillary array). Ordering information Kit Agilent HS Large Fragment 50 kb kit, 500 samples

Figure 1. Electropherogram overlay of large DNA sample sheared to different sizes and analyzed on the Agilent Fragment Analyzer system using the Agilent HS Large Fragment 50 kb kit. LM = Lower Marker and UM = Upper Marker. Figure 2. Electropherogram overlay of sheared 10 kb DNA smears at different concentrations. gDNA was sheared to a size of approximately 10 kb. Serial dilutions of this sample starting from 5 ng/µL (black trace) were prepared and analyzed on the Agilent Fragment Analyzer system, using the Agilent HS Large Fragment 50 kb kit. The overlay highlights the dynamic concentration range...