Data Sheet Ultra Sensitivity NGS Kit Agilent Femto Pulse System Femtogram-level sensitivity for unparalleled DNA analysis Using the Agilent Ultra Sensitivity NGS kit on the unique Agilent Femto Pulse system, researchers can quantify and qualify DNA smears and fragments at extremely low concentrations. Together, these solutions are ideal for the analysis of next-generation sequencing (NGS) libraries, PCR-free genomic DNA (gDNA) library preparation, shortread library quality control (QC), and cell-free DNA (cfDNA) analysis. Features and benefits – Enhanced detection Ability to visualize DNA smears down to 5 pg/µL, enabling sample conservation for downstream NGS library sequencing. – Sizing accuracy Reliable sizing from 100 to 6000 bp, making the kit ideal for applications such as short-read NGS library QC, PCR-free library preparation, and cfDNA. – Reliable quantification Visualize small RNA in a total RNA sample, including the concentration and percentage of miRNA. Ordering Information Kit Agilent Ultra Sensitivity NGS kit, 275 samples

Specifications Ultra Sensitivity NGS kit Kit Size Sizing Range Sizing Precision Sizing Accuracy Figure 1. Analysis of DNA smears and fragments at extremely low concentrations. A) An overlay of an NGS library preparation at 5 pg/µL (black trace), 10 pg/µL (blue trace), and 25 pg/µL (red trace). B) An overlay of a 300 bp DNA fragment at 50 fg/µL (black trace), 130 fg/µL (blue trace), and 500 fg/µL (red trace). Fragment Detection Range (S/N>5) Smear Detection Range 2 (S/N>5) Fragment Quantitation Range 1 (S/N>10) Smear Quantitation Range (S/N>10) 5 pg/µL per DNA fragment; 250 pg/µL total Analysis...