Data Sheet Ultra Sensitivity RNA Kit Agilent Femto Pulse System Quantify and qualify low concentration RNA for downstream applications As molecular biology advances at a breakneck pace and RNA sequencing becomes more accessible to the broader research community, confident assessment of RNA is of increasing importance. Determining RNA quality is a crucial workflow step for any researcher performing RNA-Seq and other downstream applications. The Agilent Ultra Sensitivity RNA kit, in combination with the unparalleled sensitivity of the Agilent Femto Pulse system, enables users to accurately size, quantify, and qualify very low concentration total RNA and ribo-depleted mRNA samples. To help researchers objectively measure RNA sample integrity, Agilent ProSize data analysis software offers the RNA Quality Number (RQN), a well-accepted quality metric to assess total RNA quality. With the RQN, total RNA samples are automatically scored from 1 to 10 (completely degraded RNA to fully intact RNA), providing an unbiased assessment of RNA integrity. The software also delivers an mRNA Property Summary, which indicates the percent of ribosomal RNA contamination present. Features and benefits – Reliable sizing Accurately and precisely size RNA through 6,000 nt. – Quality metrics Assess the intactness of extracted RNA with the objective RQN; percent ribosomal contamination for mRNA samples is also quantified. – Enhanced sensitivity Detect RNA at concentrations as low as 2.5 pg/µL for total RNA and 15 pg/µL for mRNA. Ordering information Kit Agilent Ultra Sensitivity RNA kit, 275 samples

Figure 1. Mouse total RNA analyzed on the Femto Pulse system with the Ultra Sensitivity RNA kit. The peak highlighted in pink corresponds to the 18S ribosomal peak, while the purple peak corresponds to the 28S ribosomal peak, respectively. The RNA Property Summary, automatically provided by ProSize data analysis software, gives the measured concentration, the ratio between major ribosomal peaks, and the RQN. LM = lower marker. 1 Figure 2. The digital gel image and table illustrate the association between RNA quality and the RQN. Identical total RNA samples (mouse) were degraded by incubation...