Genetics AZF-Microdeletions Kit - real-time PCR
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MALE FERTILITY AND Y CHROMOSOME MICRODELETIONS AZF, microdeletions of azoospermia factors, occur in the Y-chromosome regions Yq, responsible for spermatogenesis causing abnormalities in spermatogenesis and, therefore, male infertility. At molecular level, deletions extend over 0.8 - 7.7 Mb, including several genes, but the size of AZF loci is too small to be detected by cytogenetics methods, that is why “microdeletions” term is used. According to “EAA/EMQN best practice guidance for molecular diagnosis of Y-chromosomal microdeletions”, microdeletions were detected in conventional Azoospermia factors loci at the q arm of the Y chromosome, AZFa, AZFb, AZFc by real-time PCR. The absence of any group of markers in the patient specimen DNA confirms the deletion of the corresponding AZF locus. To detect AZFa, AZFb and AZFc deletions following specific marker sequences were selected: sY-84 and sY- 86 (AZFa locus); sY-127 and sY-134 (AZFb locus); sY- 254 and sY-255 (AZFc locus). The detection of the sex-determination SRY gene at the Yp arm (that is responsible for the initiation of male sex determination in humans and is absent in females) is performed in order to confirm the presence of Y- chromosome DNA in the specimen and male gender confirmation. To confirm the presence of sufficient amount of human DNA in the specimen, the amplification of HMBS gene fragment located in autosome is performed simultaneously with Y-chromosome markers. AZF-MICRODELETIONS KIT FOR BASIC DELETION SCREENING ■ The identification and analysis of Y chromosome deletions is an important research tool for studying male infertility. ■ Deletions in three regions, AZFa, AZFb, AZFc and are associated with male infertility. ■ MOLgen Genetics AZF-Microdeletions Kit amplifies nonpolymorphic short DNA segments of the Y-chromosome by using Real-Time PCR. FEATURES & ADVANTAGES ■ All-in-one dried formats, easy to use with minimized steps. ■ High potential for specific detection of all markers needed for basic deletion screening as recommended by European Academy of Andrology (EAA) and the European Molecular Genetics Quality Network (EMQN). ■ Controls: sex-determining region Y protein (SRY gene) and HMBS gene for sample quality assurance. ■ Stable storage of reagents/kits up to a year at 2-8°C, no freezing is required. ■ Transportation of kits at temperatures up to +25°C. for up to 10-days. SPECIMEN ■ Whole blood ■ Buccal epithelium AUTOMATED WORKFLOW ■ Automated Extraction - EXTRAlab ■ Automated Extraction - eLab ■ RT-PCR - AMPLilab COMPATABILITY Compatible thermal cyclers: AMPLIlab (Adaltis) CFX96™ (Bio-Rad, USA), DTprime (DT-96) Region- AZFa RELIABILITY & INNOVATION

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