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HPLC Buffers

HPLC Buffers

HPLC Buffers

Product catalog summary
Overview
ADS Biotec provides high-quality columns and buffers for scalable RNA and DNA separation and purification. Their products are designed to enhance productivity with consistent performance.

RNA/DNA Columns
  • RNASep™ Prep Column: Suitable for RNA purification for downstream biological use, offering excellent sensitivity and resolution. It is ideal for HPLC method validation before scale-up. Capacity: ~50 µg total RNA.
  • RNASep™ Semi-Prep Column: Designed for RNA purification scale-up for therapeutic use, compatible with LC instruments, and allows automation with fraction collection. Capacity: ~600 µg total RNA.
  • RNASep™ Semi-Prep II Column: Offers high-loading capacity for RNA purity assessment scale-up, ideal for 2-step purifications. Capacity: up to 2 mg total RNA.
  • DNA Purification Column: Provides superior DNA sizing and excellent sensitivity, with a robust long lifetime. Capacity: ~2.0 µg total DNA.

HPLC Buffers
ADS Biotec offers high-quality HPLC buffers essential for RNA purification, ensuring efficacy for downstream applications. They specialize in ion-paired reversed phase chromatography, providing two types of ion-pairing buffers:
  • Triethylammonium acetate (TEAA): Suitable for common nucleic acid separations.
  • Hexylammonium acetate (HAA): Developed for challenging separations, capable of resolving molecules of identical length but differing in sequence.
Custom buffer manufacturing is available, adhering to ISO 9001 Quality System standards.

Product Order Information
Various buffer solutions and columns are available in different sizes and configurations, with custom volumes and packaging options.

Contact Information
ADS Biotec Limited, Glasgow, UK, and ADS Biotec Inc., Omaha, USA, provide contact details for inquiries and orders.
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Catalog excerpts

HPLC Buffers-1

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations ADS Biotec is your source for world-class columns and buffers for the scalable separation and purification of RNA and DNA. RNA/DNA Columns Our RNASep™ line of columns are comprised of uniform alkylated nonporous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres providing high performance nucleic acid separations. Additionally, these versatile columns can be used with most HPLC\FPLC systems for many RNA and DNA separations. RNASep™ Prep Column (starter column) √ √ √ √ RNA purification for down-stream biological use RNA purity assessment Excellent sensitivity and resolution Useful for HPLC method validation prior to scale-up Catalogue Number: RNA-99-3810 7.8 x 50 mm, non-porous PS\DVB resin matrix Column capacity: ~50 µg total RNA Increased Productivity with High Quality a

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HPLC Buffers-2

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations RNASep™ Semi-Prep Column √ RNA purification scale-up for downstream therapeutic/biological use √ RNA purification for early process development and in vivo studies √ Compatible with LC based instruments √ High-loading capacity √ Ideal for 2-step purifications √ Automate with fraction collection Catalogue Number: RPC-99-2110 21.2 x 100 mm, non-porous PS\DVB resin matrix Column capacity: ~600 µg total RNA RNASep™ Semi-Prep II Column √ RNA purity assessment scale-up for downstream therapeutic/biological use √ High-loading capacity...

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HPLC Buffers-3

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations HPLC Buffers Consistent, high-quality buffers are of paramount importance for RNA purification to ensure their efficacy for downstream application and therapeutic use. Separation and purification of nucleic acids is most commonly performed using ion-paired reversed phase chromatography. This technique provides ionic interaction between the nucleic acid and the stationary phase via its hydrophobic moiety. ADS Biotec has over 20 years of experience producing the highest quality HPLC buffers available for nucleic acid separations....

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HPLC Buffers-4

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations Column Order Information Use Column Dimensions RNASep™ Prep Column Separation, purification and QC of RNA molecules RNASep™ Semi-Prep Column RNA purification for intermediate scale-up RNASep™ Semi-Prep II Column RNA purification for large scale-up DNASep™ Column High resolution ds/ssRNA and DNA analysis High resolution RNA analysis Product Description Catalogue Numbers HPLC Buffer Order Information Product Description Catalogue Numbers Mobile Phase for Nucleic Acid Analysis – Buffer A (0.1 M TEAA in water) Mobile Phase for Nucleic...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.