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mRNA Purification Solutions

mRNA Purification Solutions

mRNA Purification Solutions

Product catalog summary
Overview
ADS Biotec provides high-quality columns and buffers for scalable RNA and DNA separation and purification. Their products are designed for use with HPLC/FPLC systems and are suitable for various applications, including biological and therapeutic uses.
RNA/DNA Columns
  • RNASep™ Prep Column: Ideal for RNA purification and purity assessment, offering excellent sensitivity and resolution. Suitable for HPLC method validation. Capacity: ~50 µg total RNA.
  • RNASep™ Semi-Prep Column: Designed for RNA purification scale-up, compatible with LC instruments, and ideal for 2-step purifications. Capacity: ~600 µg total RNA.
  • RNASep™ Semi-Prep II Column: Used for RNA purity assessment scale-up with high-loading capacity. Capacity: up to 2 mg total RNA.
  • DNA Purification Column: Provides superior DNA sizing and sensitivity, with a robust and long lifetime. Capacity: ~2.0 µg total DNA.
HPLC Buffers
ADS Biotec offers consistent, high-quality buffers essential for RNA purification. Their buffers are used in ion-paired reversed phase chromatography, ensuring optimal resolution and separation consistency. Two main types are available:
  • Triethylammonium acetate (TEAA): Suitable for common nucleic acid separations.
  • Hexylammonium acetate (HAA): Developed for challenging separations, capable of resolving sequence-independent molecules.
Custom buffer manufacturing is available, adhering to ISO 9001 Quality System standards.
Product Information
Detailed catalogue numbers and specifications are provided for each product, including column dimensions and buffer compositions. Custom volumes and packaging options are available upon request.
Contact Information
ADS Biotec Limited and ADS Biotec Inc. provide contact details for inquiries and orders, with locations in the UK and USA.
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Catalog excerpts

mRNA Purification Solutions-1

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations ADS Biotec is your source for world-class columns and buffers for the scalable separation and purification of RNA and DNA. RNA/DNA Columns Our RNASep™ line of columns are comprised of uniform alkylated nonporous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres providing high performance nucleic acid separations. Additionally, these versatile columns can be used with most HPLC\FPLC systems for many RNA and DNA separations. RNASep™ Prep Column (starter column) √ √ √ √ RNA purification for down-stream biological use RNA purity assessment Excellent sensitivity and resolution Useful for HPLC method validation prior to scale-up Catalogue Number: RNA-99-3810 7.8 x 50 mm, non-porous PS\DVB resin matrix Column capacity: ~50 µg total RNA Increased Productivity with High Quality a

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mRNA Purification Solutions-2

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations RNASep™ Semi-Prep Column √ RNA purification scale-up for downstream therapeutic/biological use √ RNA purification for early process development and in vivo studies √ Compatible with LC based instruments √ High-loading capacity √ Ideal for 2-step purifications √ Automate with fraction collection Catalogue Number: RPC-99-2110 21.2 x 100 mm, non-porous PS\DVB resin matrix Column capacity: ~600 µg total RNA RNASep™ Semi-Prep II Column √ RNA purity assessment scale-up for downstream therapeutic/biological use √ High-loading capacity...

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mRNA Purification Solutions-3

RNA/DNA Columns & HPLC Buffers For Scalable Nucleic Acid Separations HPLC Buffers Consistent, high-quality buffers are of paramount importance for RNA purification to ensure their efficacy for downstream application and therapeutic use. Separation and purification of nucleic acids is most commonly performed using ion-paired reversed phase chromatography. This technique provides ionic interaction between the nucleic acid and the stationary phase via its hydrophobic moiety. ADS Biotec has over 20 years of experience producing the highest quality HPLC buffers available for nucleic acid separations....

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mRNA Purification Solutions-4

RNA/DNA Columns and HPLC Buffers For Scalable Nucleic Acid Separations Column Order Information Use Column Dimensions RNASep™ Prep Column Separation, purification and QC of RNA molecules RNASep™ Semi-Prep Column RNA purification for intermediate scale-up RNASep™ Semi-Prep II Column RNA purification for large scale-up DNASep™ Column High resolution ds/ssRNA and DNA analysis High resolution RNA analysis Product Description Catalogue Numbers HPLC Buffer Order Information Product Description Catalogue Numbers Mobile Phase for Nucleic Acid Analysis – Buffer A (0.1 M TEAA in water) Mobile Phase for Nucleic...

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