Fully Automated Immunoassay Analyzer Testing 25-OH Vitamin D is important to identify the deficiencies and to monitor supplementation TEST Principle - Two Step Sandwich Assay Vitamin D plays a vital role in calcium absorption, bone health, and potentially has benefits in various other health areas like immune system support and reducing the risk of certain diseases. Excellent precision leads to more reliable and reproducible results Eliminate the wastage often associated with opened reagent bottles Significantly shorter turnaround time - results within 30 minutes FATTY f FISH l EGGS V MILK Check with your doctor about which Vitamin D supplements are right fit for you Every 30 days Six Points 25 Minutes CLINICAL BACKGROUND [1] International Osteroporosis Foundation. [2] WHO Scientific Group on the Prevention and Management of Osteoporosis 2003 Prevention and management of osteoporosis: report of a WHO scientific group. Geneva: World Health Organization. [3] Holick MF et al. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: an Endocrine Society Clinical Practice Guideline. J Clin Endocrin Metab. 2011; 96: 1911-1930. Sample Type Sample Volume Functional Sensitivity Assay Range Limit of Detection Precision within run Calibration Frequency Calibration Level Testing time Vitamin D is a fat-soluble, secosteroid hormone precursor. In humans, the most abundant forms are Vitamin D3 (cholecalciferol), followed by Vitamin D2 (ergocalciferol), known together as calciferol. Calciferol primarily undergoes hydroxylation in the liver to 25-OH Vitamin D (calcidiol), the major circulating form of vitamin D. The serum or plasma level of 25-OH Vitamin D is recognized as the most reliable indicator of vitamin D sufficiency. A second hydroxylation in the kidneys of a small amount of 25-OH Vitamin D produces l,25(OH)2 Vitamin D (calcitriol). Calcitriol regulates calcium and phosphate blood concentrations through amongst other intestinal absorption. ◄◄Improved assay performance, offering a novel new two-step sandwich assay ◄◄Antibody that specifically recognizes immunocomplex consisting of 25-OH-D and anti-25-OH-D antibody ◄◄Alignment with LC-MS/MS gold standard and high correlation with competitive assays ◄◄No separate or additional pre-treatment steps or auxiliary solutions Although Vitamin D is commonly called a vitamin, it is not actually an essential dietary vitamin in the strict sense, as it can be synthesized in adequate amounts by most mammals exposed to sunlight. A substance is only classified as a vitamin when it cannot be synthesized in sufficient quantities by an organism, and must be obtained from a diet. Vitamin D deficiency was first discovered in the childhood disease rickets. However due to recent trends to avoid prolonged sun exposure due to cancer and aging impacts, the world’s population is even more at risk. Deficiency has been linked to various other medical conditions other than bone metabolism such as: adult osteomalacia, increased risk for fractures, secondary hyperparathyroidism, autoimmune diseases, diabetes, associated with increased risk for certain cancers, and cardiovascular problems.