Agilent 1260 Infinity HPLC-Chip/MS System – Infinitely better for microfluidic nanospray LC/MS - 12 Pages

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Agilent 1260 Infinity HPLC-Chip/MS System – Infinitely better for microfluidic nanospray LC/MS

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Infinitely better for microfluidic nanospray Agilent 1260 Infinity HPLC-Chip/MS System LO/VIO Agilent Technologies

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Agilent HPLC-Chip II and 6000 Series Mass Spectrometers Clearly better together The Agilent 1260 Infinity HPLC-Chip/MS system is based on revolutionary microfluidic chip technology specifically designed for nanospray LC/MS. The system includes Agilent 1260 Infinity Capillary and Nanoflow Pumps, High Performance Micro Autosampler with Thermostat, Chip Cube MS interface, and any Agilent 6000 Series Mass Spectrometer. System control is through either Agilent ChemStation or MassHunter software. Second generation HPLC-Chip technology incorporates a carbon ion implanted filter, improving surface...

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Phosphochip for Agilent 1260 Infinity HPLC-Chip/MS One-step phosphopeptide analysis Moving beyond protein identification, more and more researchers are focusing on the protein phosphorylation mechanism, an important post-translational modification (PTMs) process, to understand signal transduction in the cell. To investigate the phosphoproteome in more detail, phosphorylated proteins and peptides of interest need to be enriched prior to LC/MS/MS analysis due to the low abundance of phosphorylated proteins in complex mixtures. Reversed phase column 2 Reversed phase column 1 Phosphochip –...

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HPLC-Chip/MS for protein discovery to targeted quantification Protein biomarker discovery and validation Protein biomarker discovery Intensity in run 10 1e+07 Intensity: run 1 vs. run 10 Linear fit: 1.031988*x 1e+06 Correlation: 0.998783 Biomarker discovery and validation is the first step in the new paradigm of drug development involving biotechnology. Differential protein expression analysis is employed in putative protein biomarker determination in clinical research or pharmaceutical development. Featuring HPLC-Chip/MS, Agilent offers a label-free identification and relative...

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Protein biomarker validation Confirmation of putative protein biomarkers in complex biological samples requires an instrumental method that is fast, highly selective and sensitive. Peptide quantification using multiple reaction monitoring (MRM) has been established as an important methodology for biomarker validation. Easy transition from discovery to validation • The plug and play aspect of the Agilent HPLC-Chip enables the use of the same chip for discovery as well as validation thus ensuring retention integrity being retained • The shared axial acceleration collision cell between Agilent...

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Rapid identification of protein heterogeneity Analysis of monoclonal antibodies MAbs glycosylation stoichiometry The routine analysis of biopharmaceutical proteins, as well as purified proteins used in basic research, can be performed reliably with very small amounts of sample using the HPLCChip/MS system. For example, recombinant monoclonal antibodies (MAbs), which have therapeutic applications, are relatively stable biomolecules, but a number of chemical modifications and degradation reactions can occur during their manufacture, formulation and storage. Accurate mass measurement of the...

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Low level quantification in complex biological matrices Analysis of small molecules Agilent UHC-Chip with a 500 nL enrichment column. Enhanced sensitivity through UHC-Chip The Agilent ultrahigh capacity HPLC-Chip (UHC-Chip) is ideal for drug metabolism and pharmacokinetic (DMPK) studies where the volume of blood diminishes. The reduction of rodent size and number is accompanied by a cutback in the amount of the active pharmaceutical entity per PK study. This ultimately leads to substantial cost-savings in the DMPK laboratory. Small animals can only donate a limited sample volume in...

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Custom HPLC-Chip enables new research in glycan expression Case study: Boston University “The HPLC-Chip was an enabling technology for us.” Progress in biomedical research often takes a quantum leap when the right instrumentation becomes available. Glycan expression is one of those examples. Structurally diverse glycans are found on mammalian cell surfaces and in extracellular matrices. They mediate cell-cell recognition and cell-cell and cell-matrix interactions, but the exact details are largely a mystery. An innovative Agilent HPLC-Chip has now solved many of the difficulties that were...

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Powerful hair-based drug testing technology using HPLC-Chip/MS Case study: Hong Kong University “This hair-based test provides the answer to a range of technical challenges.” A Hong Kong University of Science and Technology (HKUST) professor has successfully developed a hair-based drug testing method using the Agilent HPLC-Chip/MS system with an Agilent 6410 Triple Quadrupole LC/MS. The new method is an alternative to other drug testing methods, including the urinebased method commonly used in Hong Kong. The hair-based method is 1,000 times more sensitive than the urine-based method that is...

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Agilent 1260 Infinity HPLC-Chip/MS Application examples and citations Applications A complete list of applications using Agilent HPLC-Chip/MS systems is available on our web site at www.agilent.com/chem/hplc-chip A complete list of papers from peer-reviewed proteomics publications using Agilent HPLC-Chip/MS systems is available on our web site at www.agilent.com/chem/hplc-chip Gudihal R, Ragalopalan S, Gulge R, Gieschen A, Miller C, Tang N and Waddell K, “Primary Characterization of a Monoclonal Antibody Using Agilent HPLC-Chip Accurate-Mass LC/MS Technology,” Agilent pub. no. 5990-3445EN...

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Agilent 1260 Infinity HPLC-Chip/MS Customer testimonials "Scientists should spend their time in the lab doing meaningful biological experiments, not searching for leaks and dead volumes in their nanoflow-LC system. The Agilent HPLC-Chip is the solution, making nanoflow-LC for high sensitive protein and PTM identification easy, reliable, stable, and reproducible, far exceeding the traditional setup." Dr. Manfred Raida, Experimental Therapeutic Center, Singapore “The Phosphochip is the ultimate ease-of-use tool for phosphopeptide analysis as it has a fully integrated microfluidic design that...

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