Competent Cells - 22 Pages

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Competent Cells

Catalog excerpts

Competent Cells How do you choose the right competent cell? INNOVATIVE SPECIALTY CELLS UNIQUE SOLUTIONS

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COMPETENT CELLS We have your competent cells. Finding the right competent cell for your application is easy with our extensive line of competent cells! We have innovative strains to match every cloning strategy. Our comprehensive collection of strains includes the highest transformation efficiencies available. Our specialty cells are ideal for your difficult or unusual cloning projects. Use the accompanying chart on this page to match your application to the perfect strain. DIFFICULT CLONING Large or Ligated DNA SYMPTOMS > ' No colonies after transformation ' Low representation in...

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PROTEIN EXPRESSION GENERAL CLONING General Protein Expression Toxic Proteins Generate Unmethylated DNA Convenient Cloning Unmethylated DNA SYMPTOMS > • Truncated proteins • Low levels of expression * No expression I SYMPTOMS > * No expression • Little or no bacterial growth BL21-CodonPlus® cells Universal Strain When Even Tighter Control is Required SoloPack® Gold cells (>lxl09)* #200350 SoloPack® Gold cells (>lxl08)* #200325 96Pack® Gold cells (^lxlO8)* #200324 Unlimited DNA or Subcloning ' efficiency = cfu/pg pUC18 SCSI10 cells U5xl06)* endA-for improved plasmid yield and better...

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COMPETENT CELLS Get Your Clone with Our Competent Cells From the high-efficiency ultracompetent and electroporation-competent cells to the reliable subcloning-grade competent cells, our competent cells feature the widest range of cloning efficiencies available. Whether you are cloning small amounts of DNA or doing routine day-to-day cloning, we have the right efficiency and genotype for every application. Subcloning-Grade Cells _ > 1 x 106 transformants/μg of supercoiled DNA HTE AND HEE PHENOTYPES were developed to enhance competent cell performance. These phenotypes allow you to...

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The Hte and Hee Phenotypes Large and Ligated DNA At Stratagene, we know it is more difficult to introduce large or ligated DNA constructs into competent cells than supercoiled DNA or small plasmids. We have developed the Hte (high transformation efficiency) and Hee (high electroporation efficiency) phenotypes to enhance competent cell performance for your chemical and electroporation transformations. Increased performance translates into increased success in obtaining representative primary and cDNA libraries. High Transformation Efficiency High Electroporation Efficiency The XL10-Gold®...

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COMPETENT CELLS > DIFFICULT CLONING XL10-Gold® Ultracompetent Cells Highest Efficiency Chemically Competent Cells XL10-Gold® ultracompetent cells provide the highest chemical transformation efficiencies of large plasmids and ligated DNA. The XL10-Gold strain allows cloning of methylated DNA and produces high-quality miniprep DNA. Plasmid libraries constructed in this strain are more representative because XL10-Gold cells decrease the bias against large inserts. Large DNA XL10-Gold® cells are the only chemically competent cells that potentially increase this size bias. XL10-Gold cells...

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Newly Improved Electro-Ten Blue® Cells Highest Efficiency Electroporation-Competent Cells Stratagene’s ElectroTen-Blue® electrocompetent cells offer the highest available transformation efficiencies of ≥3.0 X 1010 cfu/μg of supercoiled pUC DNA (Figure 4). High efficiency, ease-of-use, and the Hee phenotype make ElectroTen-Blue cells ideal for your most demanding cloning projects. Easily Transform Large and Ligated DNA ElectroTen-Blue® electrocompetent cells exhibit the high efficiency electroporation (Hee) phenotype. This phenotype improves the survival rate of cells, increasing cloning...

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COMPETENT CELLS > DIFFICULT CLONING Cloning Difficult DNA Unstable DNA / Toxic DNA / Methylated DNA We have created strains that solve some of the toughest cloning challenges. Our SURE® series is engineered to improve cloning of unstable DNA. The ABLE® series offers a simplified approach for propagating toxic DNA. Our MR (Restriction Minus) series is deficient in all known E. coli K12 restriction systems to eliminate cleavage of eukaryotic DNA with methylation patterns that are different than the E. coli host methylation patterns. Unstable DNA Replicating eukaryotic DNA in prokaryotic cells...

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generating mutations within the gene of interest when your protein is toxic to the cells. Try both strains to obtain the highest electroporationcompetent cells tion-competent cells. copy number that still allows growth of your construct. The ABLE strains are available as both chemically and electropora- ElectroTen-Blue® electroporationcompetent cells Methylated DNA Eukaryotic genomic DNA can be highly methylated; the methylation patterns can vary in different tissues and at different times during development. cDNA is often methylated during synthesis to protect internal restriction sites...

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COMPETENT CELLS > PROTEIN EXPRESSION Protein Expression Powerful T7 RNA Polymerase The T7 RNA polymerase-based protein expression system15 is extremely popular because it provides the highest levels of recombinant protein expression in E. coli. We offer the BL21, BL21-Gold and BL21-CodonPlus® competent cell strains specifically for use with T7 promoterdriven vectors, such as the pET and pCAL protein expression vectors. All BL21 strains are deficient in the OmpT and Lon proteases, which may interfere with isolation of intact recombinant proteins. The Problem of Codon Bias Eliminate Codon...

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POLYMERASE-BASED SYSTEMS cells EndA- cells EndA- cells EndA- cells EndA- cells EndA- strains have been optimized for expression of AT- and GC-rich genomes respectively. Use these strains when the codon usage of your sequence is known. Use the genotypes table to determine the most appropriate strain for your gene of interest. cells EndA- BL21-CodonPlus(DE3)-RIL-X and –RP-X are methionine auxotrophs for metabolic labeling of proteins for x-ray crystallography. Save 2 Days with BL21-Gold Competent Cells The BL21-Gold competent cells incorporate major improvements over the original BL21 series....

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