Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com AID EliSpot/FluoroSpot: Products and services overview 1. AID EliSpot and FluoroSpot Reader AID EliSpot Reader classic (ELR06) AID iSpot Reader (ELR06IFL) AID iSpot Reader Spectrum (ELR068IFL) AID viruSpot Reader Spectrum (VSR06IFL) AID multiSpot Reader Spectrum (MSR06) AID robotic EliSpot/FluoroSpot Reader (ELIRob06IFL) The AID EliSpot/FluoroSpot Reader software Validation on the AID EliSpot/FluoroSpot Reader Comparison of the different systems 2. AID EliSpot Assays page 2 page 5 page 8 page 11 page 14 page 18 page 20 page 22 page 23 page 24 Page 1 of 25

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com AID EliSpot Reader classic (ELR06) The basic 96‐well plate EliSpot Reader This is the classic AID EliSpot reader type. The device is fast, efficient, user‐friendly and has become the most successful EliSpot reader development in the EliSpot market. By the end of 2009 more than 400 researchers all across the continent are using this particular device. The AID EliSpot Reader classic interprets any type of EliSpot ...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Controlled by a high‐end PC‐system, comes with TFT screen, DVD burner, printer and external storage media 250 mA @ 230 V ‐ 500 mA @ 110 V One mouse click from raw data to final result meets FDA CFR 21 part 11 criteria CE (DIN EN ISO 13485, year 2003 edition) certificated Plate view of the AID EliSpot Reader software. Counted spots are shown in blue on the lower left side of each well. Upon double‐click the single well view is activated. ...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Technical specifications of the AID EliSpot System ELR06 Hardware PC system High‐end PC with Intel Core2Duo processor, 19'' screen, color printer Illumination Evenly spread long life LED ring illumination, transmitted light on demand Camera resolution and control 5 Megapixel, color, firewire‐connected Power input 250 mA @ 230 V/ 500 mA @ 110V Footprint 430x430x260 mm (Peripherals not included) Software Operating system Windows 7 Professional (32/ 64 bit) ...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com AID iSpot Reader (ELR06IFL) The basic EliSpot/FluoroSpot Reader The AID iSpot machine is one of the most successful EliSpot/FluoroSpot Reader developments in recent years. The AID iSpot system for the first time allows analyzing both: enzymatic and fluorescent (FluoroSpot) based EliSpot assays. The AID iSpot Reader comprises the same outstanding functionality, such as layout‐generator, rule‐ compiler, various export possibilities etc., as the AID ...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Controlled by a high‐end PC‐system, incl. TFT screen, DVD burner, printer and external storage media 350 mA @ 230 V ‐ 700 mA @ 110 V FluoroAID: This powerful image overlay technology allows detecting double‐stained cells where one cytokine dominates over the other. Page 6 of 25

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Technical specifications of the AID iSpot Reader (ELR06IFL) Hardware PC system High‐end PC with Core2Duo processor, 19'' screen, color printer Fluorescent filter set and control 2 narrow‐banded filters on board, 4 position filter/ LED changer "FluoroAID", AID's patented well overlay technology Fluorescent imaging Illumination Evenly spread, long life LED ring and external Xenon light, transmitted light on request demand Camera resolution and control...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com AID iSpot Reader Spectrum (ELR068IFL) The ultimate high resolution EliSpot/FluoroSpot Reader The iSpot Spectrum FluoroSpot Reader is the newest generation of the successful AID iSpot, the first commercially available combined EliSpot/ FluoroSpot Reader. The iSpot Spectrum is equipped with an 7&1 position filter wheel, which allows for a customized selection of up to 7 individual narrow band fluorescent filters, whilst ...

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Three color fluorescent assay. Output of a FluoroSpot assay. Green signal (IFN‐y), red signal (IL‐2), the corresponding overlaid image and the resulting picture showing only double‐stained spots. The distance between spots where a double/triple stained spot is detected can be defined by each user. Page 9 of 25

Autoimmun Diagnostika GmbH Ebinger Str. 4 D-72479 Strassberg Germany Tel. +49-743493640 Fax +49-7434936440 www.aid-diagnostika.com Technical specifications of the AID iSpot Spectrum Reader (ELR068 IFL) Hardware PC system Fluorescent filter set and control Fluorescent imaging High‐end PC with Core2Duo processor, 19'' screen, color printer 3 narrow‐banded filters on board, 8 position filter/ LED changer "FluoroAID", AID's patented well overlay technology Illumination Evenly spread, long life LED ring and external Xenon light, transmitted light on request demand Camera resolution and control...