SIDECAR
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FIRST AUTOMATED STREAKING SYSTEM INTEGRATED WITH THE RAPID BACTERIA CULTURE THE FIRST REAL WALK-AWAY SYSTEM! Alifax presents the first instrument in the world totally automated for rapid bacterial growth integrated to a streaking system. The system is composed of two units: Alfred60 AST and Sidecar. Thanks to the Light Scattering technology, Alfred60 AST detects the presence of bacteria and their resistance to drugs in a few hours with high sensitivity and specificity. Alfred60 AST monitors the growth phases of bacteria from the inoculum step into specific culture broths providing real time growth curves and quantitative bacterial results in CFU/ml. Broth turbidity level is detected by the Mc Farland Monitor and as the sample reaches the 0.5 Mc Farland it is buffered into the refrigerated area where it is ready to be tested with a customized antibiotic panel. WindowsTM operative System TESTS AND APPLICATIONS Urine culture 3 hours cutoff 30.000 CFU/ml Sidecar is the automated streaking system able to stock 240 Petri dishes chosen among a maximum of 12 different media. Residual Antimicrobial Activity (RAA) test Simultaneously to the culture test The streaked dishes are incubated at 37°C for the requested analysis time in a incubator included in the system. Bacteria Culture on special sample* In the main operating method only the positive samples are streaked automatically. The advantages are the following: • Negative samples are reported in one day • Drastic reduction of streaked samples • Material saving • Reading time saving • Reduction of the use of broad-spectrum antibiotics • Decrease of the spread of bacterial resistance. In addition, all the applications currently offered by Light Scattering technology are kept unchanged thus bacterial culture of Biological Fluids and MRSA screening can run simultaneously in 6 hours and the Susceptibility testing in 3 hours. Human Biological Liquid Bacteria Culture* 6 hours cutoff <50 CFU/ml 6 hours cutoff <50 CFU/ml Automated Mc Farland Monitor Susceptibility testing with customized antibiotic panel for: • Urine • Human Biological Liquids • Positive Blood Culture • Isolated Colonies FAST PROTOCOL STANDARD PROTOCOL (URINE ONLY) (URINE or HBL) THRESHOLD (CFU/ml) THRESHOLD (CFU/ml) 1.000.000 500.000 100.000 DEFAULT 50.000 15.000 10.000 - Windows is a Microsoft trademark

Light Scattering Technology Quantitative results expressed in CFU/ml Automated susceptibility testing with customized antibiotic panels Real time detection of bacteria growing curves Automatic reagents and samples dispensing Continuous loading of primary closed tube Automatic results reading and reporting Built-in barcode reader for automatic sample identification LIS bidirectional interface and Query Host application Connection to HB&L Refrigerated area at + 4°C for the storage of primary samples, antibiotics and 0.5 Mc Farland positive samples Refrigerated stocking area for 240 petri...