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6-Sulfatoxymelatonin ELISA
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Catalog excerpts

6-Sulfatoxymelatonin ELISA - 1

6-Sulfatoxymelatonin ELISA Immunoassay for the quantitative determination of 6sulfatoxymelatonin in human urine. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: Version:

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6-Sulfatoxymelatonin ELISA - 2

The ALPCO 6-Sulfatoxymelatonin ELISA is intended for the quantitative determination of 6-sulfatoxymelatonin in human urine samples. For Research Use Only. Not for use in diagnostic procedures. PRINCIPLE OF THE ASSAY Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of...

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Precision pipettes for dispensing up to 1000 μL (with disposable tips) Repeating or multi-channel pipette for dispensing up to 1000 μL Volumetric containers and pipettes for reagent preparation Distilled/Deionized water for reagent preparation Microplate washer or wash bottle Microplate shaker capable of 700-900 rpm Microplate reader Centrifuge (1,000 x g to 3,000 x g) Vortex for sample preparation PRECAUTIONS 1. For research use only. Not for use in diagnostic procedures. 2. Before starting the assay, read the instructions completely and carefully. Use the valid version of the package...

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17. Microtiter plate washing is important. Improperly washed wells will give erroneous results. It is recommended to use a multichannel pipette or an automatic microtiter plate washing system. Do not allow the wells to dry between incubations. Do not scratch coated wells during rinsing and aspiration. Rinse and fill all reagents with care. While rinsing, check that all wells are filled precisely with Wash Buffer, and that there are no residues in the wells. 18. Humidity affects the coated wells/tubes. Do not open the pouch until it reaches room temperature. Unused wells/tubes should be...

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QUALITY CONTROL Control samples should be analyzed with each run. Results generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits. ASSAY PROCEDURE Gently mix all reagents before use. 1. Pipette 10 µL of each Calibrator, Control and urine sample into polystyrene, polypropylene or glass tubes. Avoid direct sun light. 2. Pipette 500 µL of Assay Buffer into each tube. Vortex....

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PERFORMANCE CHARACTERISTICS Analytical Sensitivity LOD: 1.0 ng/mL Typical Calibration Curve (Example. Do not use for calculation) Melatonin Sulfate (ng/mL) Range (ng/mL) Dilutional Linearity Range (ng/mL) Serial dilution up to Range (%) 1:32 Spiking Recovery Mean recovery after spiking (%) Range (%) 105.8

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Cross-reactivity of other substances tested < 0.0001 %

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