GENERAL ITEMS - ALZET Osmotic Pumps* - Homeothermic pad - Sterile drape - Sterile gauze, cotton swabs - Hair clippers (#40 blade) - 70% ethanol - Betadine solution - Glass bead sterilizer - Ocular lubricant - Wound Clips (7mm for mice, 9mm for rats)* - Wound Clip Applier* - Wound Clip Remover* - Scalpel handle #3 - Scalpel blade #15 - Brown Adson forceps - Hemostat (straight tip) - Sutures (absorbable, 4.0) * Available from DURECT Corp. ([email protected]) _✓ ALZET pumps can be implanted intraperitoneally in animals with sufficiently large peritoneal cavities. With any substance administered intraperitoneally, whether by injection or by infusion, a majority of the dose may be absorbed via the hepatic portal circulation rather than by the capillaries. For substances that are extensively metabolized by the liver (i.e., have a high “first pass effect”), the intraperitoneal route of administration may produce highly variable concentrations of agent in plasma and consequently highly variable effects. Therefore, the intraperitoneal route should probably be avoided with agents that have a significant first-pass effect. Pump Preparation (refer to the package insert for complete filling instructions) - Fill the empty ALZET pump with your vehicle or drug solution using a syringe and filling tube according to the procedure listed in the package insert included in your box of pumps. - Insert the flow moderator into the filled ALZET pump until the cap or flange is flush with the top of the pump. - Prime the filled pumps in sterile saline at 37°C. - Refer to your package insert for the appropriate period of priming. Most pumps require at least an overnight priming period. Anesthesia Anesthesia is required for surgical implantation of ALZET pumps. - Anesthetize the animal using either an inhalable (i.e., Isoflurane) or injectable (i.e., Xylazine® and Ketamine®, or sodium pentobarbital) anesthetic. alzet OSMOTIC PUMPS SSIP-7.14 DURECT Corporation | 10260 Bubb road | Cupertino CA, 95014-4166 T: 408.367.4036 F: 408.865.1406 Toll Free 800.692.2990 WWW.ALZET.COM | [email protected]

ALZET SURGICAL INSTRUCTION SHEET The use of inhalation anesthetics, such as Isoflurane, is highly recommended. It supplies supplemental oxygen during periods of respiratory depression and provides for rapid anesthetic recovery. Surgical Preparation - Apply ocular lubricant to the eye. - Shave the area centered over the site chosen for pump placement. - Using a sterile swab, disinfect the incision site with 70% ethanol by working outwardly in ever widening circles. With a new sterile swab, apply iodine over the proposed incision site in a similar fashion. Repeat the ethanol and iodine scrub one...