GENERAL ITEMS DURECT Corporation | 10260 Bubb road | Cupertino CA, 95014-4166 T: 408.367.4036 F: 408.865.1406 Toll Free 800.692.2990 WWW.ALZET.COM | [email protected] - ALZET Osmotic Pumps* - Homeothermic pad - Sterile drape - Sterile gauze, cotton swabs - Hair clippers (#40 blade) - Ethanol or chlorhexidine - Betadine scrub - Glass bead sterilizer - Ocular lubricant - Syringe w/ sterile saline 7mm Wound Clips* Reflex Wound Clip Applier* Reflex Wound Clip Remover* Scalpel handle #3, blade #15 (or Iris scissors) Moria forceps (smooth) Dumont forceps (#5-45) Hemostat (straight tip) Syringe needle (22-20 gauge) Scissors (fine Iris or Vannas spring) Sutures (non-absorbable, 6-0) * Available from DURECT Corp. (877-922-5938, [email protected]) J - ✓ Pump Preparation (refer to the package insert for complete filling instructions) - Fill the empty ALZET pump with your vehicle or drug solution using a syringe and filling tube according to the procedure listed in the package insert included in your box of pumps. - Insert the flow moderator into the filled ALZET pump until the cap or flange is flush with the top of the pump. - Prime the filled pumps in sterile saline at 37°C. - Refer to your package insert for the appropriate period of priming. Most pumps require at least an overnight priming period. Anesthesia Anesthesia is required for surgical implantation of ALZET pumps. - Anesthetize the animal using either an inhalable (i.e., Isoflurane) or injectable (i.e., Xylazine® and Ketamine®, or sodium pentobarbital) anesthetic. The use of inhalation anesthetics, such as Isoflurane, is highly recommended. It supplies supplemental oxygen during periods of respiratory depression and provides for rapid anesthetic recovery. Surgical Preparation - Apply ocular lubricant in the eye. - Remove fur from area centered over the ventral neck area. Loose hair can be removed with dry gauze, gauze dampened w/ ethanol or adhesive tape. - Disinfect the skin first with 70% ethanol. Using a sterile swab apply the ethanol at the proposed incision site and work outward in ever widening circles over the entire hair removed area. With a new sterile swab, apply iodine at the proposed incision site and again work outward in ever widening circles. Repeat the ethanol and iodine scrub. - Administer analgesic as directed by staff veterinarian Surgical Procedure - Place the rodent in dorsal recumbency with the head secured, facing toward the surgeon. (For ease of manipulation during surgery, the animal can be placed in a sterile stockinette and the head and neck exposed for anesthesia administration and surgical access.) - Place a small bolster beneath the animal's neck to expose the ventral neck more fully, alzet OSMOTIC PUMPS SSIVM - 6.15

MOUSE INTRAVENOUS CANNULATION MOUSE JUGULAR CATHETER ADJUSTABLE LENGTH (#0007701) - Use a small, sharp scalpel blade or Iris scissors to make a single 7-9 mm skin incision from the ramus of one side of the jaw to the clavicle just lateral to the trachea/midline. - Gently dissect down through the adipose tissue and fascia to isolate the external jugular vein, which is superficial to most of the neck musculature. Tunnel under the vessel by gently lifting the vein with the Moria forceps and clean the jugular vein. - Place one loose ligature around the cardiac end of the vein and another ligature...