SUBCUTANEOUS IMPLANTATION GENERAL ITEMS
 - ALZET Osmotic Pumps*
 - Homeothermic pad
 - Sterile drape
 - Sterile gauze, cotton swabs
 - Hair clippers (#40 blade)
 - 70% ethanol
 - Betadine solution
 - Glass bead sterilizer 
 - Ocular lubricant ALZET SURGICAL INSTRUCTION SHEET Pump Preparation 
 (refer to the package insert for complete filling instructions) - Fill the empty ALZET pump with your vehicle or drug solution using a syringe and filling tube according to the procedure listed in the package insert included in your box of pumps. - Insert the flow moderator into the filled ALZET pump until the cap or flange is flush with the top of the pump. - Prime the filled pumps in sterile saline at 37°C. - Refer to your package insert for the appropriate period of priming. Most pumps require at least an overnight priming period. SURGICAL TOOLS
 - Wound Clips (7mm for mice 9mm for rats)*
 - Wound Clip Applier*
 - Wound Clip Remover*
 - Scalpel handle #3
 - Scalpel blade #15
 - Brown Adson forceps
 - Hemostat (straight tip) Anesthesia is required for surgical implantation of ALZET pumps. - Anesthetize the animal using either an inhalable (i.e., Isoflurane) or injectable (i.e., Xylazine® and Ketamine®, or sodium pentobarbital) anesthetic. The use of inhalation anesthetics, such as Isoflurane, is highly recommended. It supplies supplemental oxygen during periods of respiratory depression and provides for rapid anesthetic recovery. Surgical Preparation * Available from DURECT Corp. - Apply ocular lubricant to the eye. - Shave the area centered over the site chosen for pump placement. DURECT CORPORATION | 10260 BUBB ROAD | CUPERTINO CA, 95014-4166 T: 408.367.4036 F: 408.865.1406 TOLL FREE 800.692.2

SUBCUTANEOUS IMPLANTATION ALZET SURGICAL INSTRUCTION SHEET 
 - Close the incision with wound clips (7mm for mice, 9mm for rats). Two clips will normally suffice. Post Operative Analgesia
 - An analgesic can be given post-operatively as needed. Analgesic treatment should be provided under the direction of the staff Veterinarian. AUTOCLIP KIT 500 9mm WOUND CLIPS (ITEM #0009954) Clinical Monitoring and Management
 - Animals should be monitored daily until the wound clips or sutures are removed then once to twice weekly until completion of the study. - Using a sterile swab, disinfect the incision...