Ampliqon PCR Enzymes Catalogue
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PCR ENZYMES Polymerases Buffers Nucleotides Accessories

ABOUT THIS CATALOGUE Dear reader, The purpose of this Ampliqon PCR enzyme catalogue is to offer you a convenient overview of our PCR enzymes. Ampliqon enzymes include a wide range of highly pure enzyme kits suitable for all DNA amplification purposes. They are characterised by robust performance, high stability and no contaminating activities. In the catalogue you find selection charts, stability guidelines, practical information on our proprietary buffer systems and a section on available PCR accessories. Included you will also find our general terms of sale and delivery. We hope that you...

Ampliqon is ISO 9001 certified, which give our customers some important benefits. The focus areas of ISO 9001 are the customers, customer satisfaction, continuous improvements of the processes and of the quality management system. We are always delighted to participate in sales support seminars and training sessions that benefit the activities of our end-user customers, the scientific communities and distributors. Website On our website ampliqon.com, you can find even more information about the products. From the user-friendly Download Center, you can download datasheets, product sheets,...

Standard PCR 6 Introduction 8 Taq DNA Polymerase 9 Taq DNA Polymerase RED 10 Introduction to master mixes 11 Taq DNA Polymerase Master Mix 12 Taq DNA Polymerase Master Mix RED - for direct loading 14 Taq OptiMix CLEAR Hot start 17 Introduction 18 TEMPase Hot Start DNA Polymerase 21 Introduction to master mixes 22 TEMPase Hot Start DNA Polymerase Master Mix 23 TEMPase Hot Start DNA Polymerase Master Mix BLUE - for direct loading Glycerol Free Products 24 Introduction 26 Taq DNA Polymerase Glycerol Free 27 TEMPase Hot Start DNA Polymerase Glycerol Free 28 PCR Buffers High fidelity 30 AQ90...

Introduction Ampliqon Taq DNA Polymerase is an excellent thermostable Taq DNA polymerase because of its high performance. Ampliqon Taq DNA Polymerase is stable and reliable, shows no contaminating nuclease activities, and each batch production offers same robust performance. Taq DNA Polymerase is the perfect match for routine PCR applications that require high yield and reliable DNA amplification. Ampliqon Taq DNA Polymerase has a molecular weight of approximately 95 kDa and exhibits 5'→3' DNA polymerase activity and 5'→3' exonuclease activity. The 5'→3' exonuclease activity leaves 3'dA...

THE ORIGIN OF AMPLIQON TAQ DNA POLYMERASE Ampliqon Taq DNA Polymerase originates from the thermophilic bacterium Thermus aquaticus, which was first discovered in hot springs in Yellowstone National Park, USA, in the 1960s. Taq DNA polymerase was the first heat-stable enzyme ever isolated, and it formed the basis for the future Nobel Prize-winning PCR technology. Later, a variety of other heat-stable enzymes were isolated and some also became commercially available. Fortunately, Taq was among the first enzymes to be discovered and is commonly agreed to be one of the best polymerases available....

Ampliqon Taq DNA Polymerase is popular because of its robust and consistent performance. Ampliqon Taq DNA Polymerase is suitable for routine PCR applications that require high yield and reliable DNA amplification. Without buffer Product number A110003 Features High product yield With 10x Ammonium Buffer dUTP incorporation possible Standard testing Routine PCR Screening With 10x Standard Buffer and MgCl2 Product number A112103 High throughput testing Did you know? Taq DNA Polymerase is tolerant to incubation at elevated temperatures for long periods without losing activity: at 40 °C for 2...

Without buffer Product number A200003 Ampliqon Taq DNA Polymerase RED provides convenient identification of enzyme addition to the tube and confirmation of complete mixing. The product includes an inert red dye that does not interfere with the PCR reaction but adds visibility to the enzyme. This makes it especially useful for high throughput testing. With 10x Ammonium Buffer and MgCl2 Features Easy identification of enzyme addition Confirmation of complete mixing High product yield Processes up to 5 kb dUTP incorporation possible Leaves a 3'dA overhang With 10x Standard Buffer and MgCl2 Product...

Taq DNA Polymerase master mixes AMPLIQON llll ID No : Lot No Introduction Ampliqon Taq DNA Polymerase master mixes are time-saving alternatives to Taq DNA polymerase kits. Fewer reagent handling steps significantly reduce set-up time and eliminate the risk of contamination of stock solutions. Furthermore, fewer handling steps lead to increased reproducibility, which makes Taq DNA Polymerase master mixes suitable for standard tests. Taq DNA Polymerase master mixes are ready-to-use master mixes. Just add your template and primers to successfully carry out PCR. Taq DNA Polymerase master mixes...

Product number A140301 100 reactions Product number A150301 100 reactions Taq DNA Polymerase Master Mix is a timesaving alternative to Taq DNA Polymerase. Taq DNA Polymerase Master Mix is excellent for robust and reliable PCR as it offers the same eminent performance as Taq DNA Polymerase. Features Time-saving premixed solution Increased reproducibility Minimal optimisation High product yield dUTP incorporation possible Processes up to 5 kb Leaves a 3'dA overhang Suitable for Standard testing and routine PCR Screening High throughput testing

Taq DNA Polymerase Master Mix RED For direct loading Taq DNA Polymerase Master Mix RED allows you to load your PCR products directly onto the agarose or SDS DNA gel after DNA amplification. There is no need for a separate loading buffer and no timeconsuming sample preparation before electrophoresis. This makes Taq DNA Polymerase Master Mix RED especially suitable for high throughput standard tests. 2x master mix, 1.5 mM MgCl2 final Product number 100 reactions Taq DNA Polymerase Master Mix RED includes a red dye and stabiliser. These do not interfere with the PCR. Taq DNA Polymerase Master...

Best-selling product Taq DNA Polymerase Master Mix RED is without doubt the most preferred PCR solution. The popularity of Master Mix RED is due to the user-friendly experience and the convincing and reliable PCR results obtained. The Master Mix RED can be used for all standard PCR applications. Ampliqon has developed two user-friendly applications using Taq DNA Polymerase Master Mix RED. Sanger sequencing Add PureIT ExoZAP PCR CleanUp to remove primers and dNTP’s Despite the red colour within Taq Master Mix RED, the obtained amplicons can easily be used for Sanger sequencing. Simply add...