ANDOR an Oxford Instruments company Specifications Summary Andor TuCam - High Performance, Two Camera Imaging Adapter Andor’s TuCam is a new generation two-camera adapter for macro or microscopic imaging applications. Available in C- or CSU-X-mount, TuCam features include large aperture, exceptional transmission, very low distortion and high precision alignment using kinematic cassettes. TuCam can be configured for simultaneous imaging from two similar cameras or as a switch between camera models with different imaging capabilities. A full range of beam splitting optics are available with custom-designed kinematic cassettes for precision alignment. These include wavelength and polarization splitters of the highest quality as well as a first surface mirror for switching between cameras. A variety of camera tubes and lenses is available to provide magnifications of 1.0x, 1.2x, 1.5x and 2.0x indepently for each arm of the adapter. A filter wheel can also be integrated at the input of TuCam to enable pre-filtering of the desired emission band. Wavelength range Application Guide s Real time multi color imaging s Co-localization s Fluorescence Resonance Energy Transfer (FRET) s Ratiometric imaging s Super resolution s Anisotropy imaging (inc. homo-FRET) Features and Benefits • Largest aperture Unique 22 mm aperture for large format sensors e.g. Neo and Zyla sCMOS • High quality achromatic lenses Image from 425 - 700 nm with minimal adjustment • Highest transmission > 96% @ 425 - 700 nm • Very low distortion < 0.5% • Bypass mode Dovetail mount for precise insertion, exchange and bypass of optical elements • Robust, compact and accurate Rigid structure provides optical and mechanical stability • Convenient user adjustment User-controls for focus adjustment and 2-axis cassette alignment are accessed via the front porch • C-mount and CSU versions Couple directly to filter wheels, microscopes, C-lenses and spinning disk confocal scanners • Various magnifications Match cameras to CSU aperture or control effective pixel size s Biplane / dual focal plane imaging s Calcium flux / ion signalling e.g. Fura, Fluo-3 dyes s Dual wavelength TIRF microscopy s Dual wavelength real-time confocal microscopy s Fluorescence In Situ Hybridization (FISH) imaging s Simultaneous fluorescence /DIC imaging

Two Camera Imaging Adapter ANDOR an Oxford Instruments company For detailed notes on performance figures annotated above, please refer to the last page of this specifications sheet. CSU-X version C-mount version

1024 x 1024 sensor, 26 fps Large Field of View EMCCD Ultimate Speed and Sensitivity Two Camera Imaging Adapter Dichroics and Polarizing Beams Splitters Moxtek® The graphs above show the transmission and reflectance curves for Semrock imaging dichroic beamsplitters and Moxtek optically flat polarizing beamsplitters. Both types of beamsplitter are optimized for use at a 45° angle of incidence. Semrock’s beamsplitters efficiently separate multicolored emission signals while maintaining excellent image fidelity. These dichroic beamsplitters are available for many popular fluorophore pairs. Their...

Two Camera Imaging Adapter ANDOR an Oxford Instruments company Creating The Optimum Product for You TR-DCI(X - SUT) example shown Step 1. Choose the mounting type Simply select from the 2 mounting options that best suit your needs: Optical Cassettes Step 4. Choose Wavelength or Polarization Filter Sets Short Description Wavelength & Polarization Filter Sets

Two Camera Imaging Adapter ANDOR an Oxford Instruments company Step 4. Choose Wavelength or Polarization Filter Sets (continued from page 4) Short Description Wavelength & Polarization Filter Sets The optical height of the system is 110 mm. We recommend that cameras and microscopes are raised using one of the following accessories: Description The following accessory is also available to enable Nomarski DIC imaging and user alignment of new dichroics with the calibration slide: Internal mounting for DIC polarizer TR-DCIS-DIC-MNT Note: We minimize chromatic aberrations in our systems, but other...

1024 x 1024 sensor, 26 fps Large Field of View EMCCD Ultimate Speed and Sensitivity Two Camera Imaging Adapter Product Drawings Dimensions in mm [inches] CSU-X version Weights: Cassette = 0.35 Kg [12 oz] Main unit = 5.5 Kg [12 lb 2 oz] C-mount version Weights: Cassette = 0.35 Kg [12 oz] Main unit = 4 Kg [8 lb 13 oz] Recommended Microscopy Software For TuCam The following software packages have been verified under simultaneous dual camera acquisition mode, as well as offering functionality to merge and analyze data from each channel. Please see ‘Application and Technical Notes’ section of the...

1024 x 1024 sensor, 26 fps Large Field of View EMCCD Ultimate Speed and Sensitivity Two Camera Imaging Adapter Order Today Need more information? At Andor we are committed to finding the correct solution for you. With a dedicated team of technical advisors, we are able to offer you one-to-one guidance and technical support on all Andor products. For a full listing of our regional sales offices, please see: andor.com/contact Our regional headquarters are: Europe Japan Belfast, Northern Ireland Tokyo Phone +44 (28) 9023 7126 Phone +81 (3) 6732 8968 Fax +44 (28) 9031 0792 Fax +81 (3) 6732 8939 North...