https://andor.oxinst.com/products/multi-wavelength-imaging/optosplit-ii
3Pages

One Camera Solution Dual Emission Image Splitter 1024 x 1024 sensor, 26 fps Large Field of View EMCCD Ultimate Speed and Sensitivity Multi-Wavelength Imaging Dual Emission Image Splitter • Single camera Cost effective - only one camera required The Optosplit II image splitter is a simple and elegant device for dividing an image into • Variable internal path separation Minimizing the introduction of aberrations a single camera chip. • Dichroic mirror and emission filters mounted in a readily interchangeable cube Exchange filter sets both easily and quickly. Some competing products have factory fitted filters • Variable and locking rectangular diaphragm aperture for defining field size Define the ROI both horizontally and vertically and set the images to the optimum size for the camera sensor • Compact design with integral C-mount input and output ports Integral C-mounts allow it to be easily attached to a wide variety of standard microscopes and CCD cameras • Simple and precise controls for image registration Split images can be accurately and easily centred in the desired field of view, and pixels aligned with respect to each other • Interchangeable filter/dichroic holders for dual and single wavelength imaging Flexibility to use multiple wavelengths by simply changing filter and re-sizing the defined field • Aperture diaphragms to balance signal levels if appropriate Acts as an adjustable neutral density filter, which can be more convenient than using neutral density filters • Rotating filter mount for polarization studies Accurately orientates the emission polarization to maximize the contrast two separate, spatially equivalent, components that can be displayed side by side on Splitting is usually performed on the basis of wavelength, allowing applications such as ratiometric calcium imaging or FRET, however, polarizing beamsplitters are also supported. The two images can be captured simultaneously offering a major benefit over manual or electronic filter changers. A rectangular aperture is used to define the region to be imaged, with a set of simple controls allowing the user to vary the relative positions of the two output images on the camera. The Optosplit II can significantly widen the scope of any fluorescence imaging system. The Optosplit II includes controls to allow the two images to be positioned accurately and conveniently within the camera frame. Images can be acquired using any imaging software and processed either manually off-line or using an appropriate analysis tool such as the Splitview module in MetaMorph (Molecular Devices) or Field Split in Andor iQ. Key Applications Ratiometric calcium and pH imaging Dual probe imaging Fluorescence Resonance Energy Transfer (FRET) Total Internal Reflectance Fluorescence (TIRF) Polariza

One Camera Solution Dual Emission Image Splitter an Oxford Instruments company Step 1. Choose the Optosplit II using the following product code: TR-OPTS-20B 1.0x magnification. TR-OPTS-20B suits the larger sensor size of the Zyla 4.2, Zyla 5.5, Neo 5.5 and iXon Ultra 888. This Optosplit II is suitable for all imaging cameras from Andor, ‘one size fits all’. How to customize the Optosplit II: [ Step 1. ] Select the Optosplit II product code. [ Step 2. ] Please indicate the filter set you require. ^ Step 3. ] Please indicate which accessories are required. Items shipped with your Optosplit II...

1024 x 1024 sensor, 26 fps Large Field of View EMCCD Ultimate Speed and Sensitivity One Camera Solution Dual Emission Image Splitter Product Drawings Recommended Software Device drivers are included in several commercial imaging packages to assist registration and to allow real-time and off-line ratioing or fluorescence overlays. Alternatively, the Optosplit can be used with simple image capture software and the processing carried out manually off-line. The simple and accessible design makes the Optosplit an excellent platform for alternative applications, such as dual polarization imaging....