Electrophoresis CELLULOSE ACETATE MEMBRANES & ELECTROPHORESIS SUPPLIES
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APACOR Performance Benefits • Range of membrane sizes • Clinical and routine analysis • Can be used in all machines • High sensitivity • Easy to handle • Clear resolution • Reproducible results Principle Hb Differentiation The membrane is supplied in pre-cut dimensions for immediate use in all electrophoresis apparatus. Apacor supply a comprehensive range of ancillary products including, buffers, reagents and stains which have been optimised for use with Apacor Cellulose Acetate Membranes. The electrophoretic separation and detection of haemoglobins is a principal application for Apacor Cellulose Acetate Membranes. The membrane provides unrivalled clarity for the diagnosis of haemoglobinopathies including Sickle Cell Diseases and Thalassemia. Electrophoresis exploits the negative charge which haemoglobin will adopt under alkaline conditions; since each haemoglobin variant carries a different net charge, they will all migrate at different speeds. Following electrophoretic migration these fractions are visualised by staining and classified by comparison with known standards. Haemoglobinopathies occur when production of normal adult haemoglobin is suppressed and replaced by one or more variants, or normal haemoglobinsare produced in abnormal proportions. Screening can highlight these abnormalities before the onset of symptoms, allowing better patient management and counselling. Procedure Overview Serum Electrophoresis Apacor Cellulose Acetate Membranes are indicated for use in the electrophoretic separations of serum proteins. This process is the single most powerful tool to indicate the wellbeing of a patient, with a large spectrum of diseases indicated by atypical protein migration. Electrophoretic separation exploits the speed of migration as determined by the protein charge. Atypical protein bands indicate clinical significance.

CELLULOSE ACETATE MEMBRANES & ELECTROPHORESIS SUPPLIES STEP 1 - SAMPLE APPLICATION Allow the membrane to soak fully using Apacor High Resolution Buffer II. Dry with adsorbent pads and then place the applicator block onto the membrane. Load a 25µl sample in a 15 second application. STEP 2 - ELECTROPHORESIS Ensure that the electrophoresis chamber is filled with High Resolution Buffer II and that the polarity of the chamber is correct. Electrophorese for 20 minutes at 2-10 milliamps. STEP 3 - STAINING Remove the membrane from the electrophoresis chamber and transfer to vessel containing Ponceau S...