CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS

Product catalog summary

Overview: The document provides a detailed description of the Chirascan and Chirascan V100 systems, which are versatile research tools designed for high-performance circular dichroism (CD) analysis. These systems are used to determine structural and thermodynamic properties of biomolecules, offering insights into secondary and tertiary structures, and their responses to thermal or chemical changes.

Key Features:

Structural and Thermodynamic Analysis: The systems allow for the detection of changes in secondary and tertiary structures, and the determination of thermodynamic properties through continuous thermal ramping. This includes monitoring at each wavelength and deriving melting points and enthalpies for multiple thermal transitions.
Sensitivity and Accuracy: The Chirascan V100 uses an optics-based, multiwavelength calibration method to ensure accurate CD values across the entire wavelength range. It features an avalanche photodiode detector for enhanced sensitivity and increased signal-to-noise ratio compared to conventional methods.
Data Quality: The systems are equipped with features such as built-in temperature control and a variety of cuvettes to ensure high-quality data acquisition. They also include a photoelastic modulator, monochromator, and an air-cooled xenon lamp for precise measurements.
Sample Optimization: A range of cuvettes and holders are available to optimize sample concentration and absorbance, crucial for acquiring high-quality data. These are made from far-UV quartz and are designed for both secondary and tertiary structure analysis.
Accessories and Capabilities: The systems can be expanded with dedicated accessories such as a CCD fluorometer, titrator and pH probe, 6-cell turret, and more. These accessories enhance the system's capabilities, allowing for the monitoring of fluorescence changes, concentration- and pH-dependent changes, and the study of solid-state samples.

Applications: Chirascan systems are widely used in research areas requiring detailed analysis of biomolecular structures and their interactions. They are particularly useful for studying protein folding, ligand binding events, and the effects of environmental changes on molecular structures.

Catalog excerpts

CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-1

CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS HIGH PERFORMANCE, READY TO RUN • Determine structural and thermodynamic properties - Gain insight and detect changes in secondary and tertiary structure - Determine response to thermal or chemical changes - Study folding and unfolding mechanisms • Achieve highest sensitivity and accuracy • Generate highest quality data • Optimize sample concentration and absorbance • Expand capabilities with dedicated Chirascan accessories Discover when change is sign

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-2

DETERMINE STRUCTURAL AND THERMODYNAMIC PROPERTIES Gain insight and detect changes in secondary and tertiary structure Secondary structure: far-UV spectrum of a globular protein Tertiary structure: near-UV spectra of two monoclonal antibodies combined contributions from a-helix and β-sheet Peak positions Circular dichroism / mdeg. Circular dichroism / mdeg. Simultaneous acquisition of CD and absorbance spectra, 0.5 mm pathlength, Chirascan V100. Courtesy of leading research university, Germany Differences between near-UV spectra due to slight changes in orientation of aromatic moieties, Chirascan...

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-3

ACHIEVE HIGHEST SENSITIVITY AND ACCURACY Since their introduction in 2005, Chirascan™ systems have continued to feature in thousands of peer-reviewed publications covering a wide range of research areas. Chirascan V100 now offers the increased sensitivity and accuracy preferred for CD analysis of biomolecules. • Avalanche photodiode detector enhances sensitivity • Increased signal:noise compared to conventional photomultiplier • Accurate normalization from simultaneous measurement of absorbance and CD Increased sensitivity when sample is limited Increased sensitivity: faster measurements for...

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-4

READY TO RUN – GENERATE HIGHEST QUALITY DATA Chirascan systems are supplied with features and accessories required for acquisition of high quality CD data – from built-in temperature control during analysis to cuvettes for the most common analytical conditions.* A basic training program follows installation to familiarize users new to Chirascan. AVALANCHE PHOTODIODE DETECTOR (CHIRASCAN V100) • Highest sensitivity (high signal: noise) PHOTOELASTIC MODULATOR PHOTOMULTIPLIER DETECTOR (CHIRASCAN) • Converts horizontally polarized light to circularly polarized light. Alternates between left- and right-handed...

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-5

CONTROL AND ANALYSIS SOFTWARE TEMPERATURE-CONTROLLED SAMPLE CHAMBER • Consistent analytical conditions • Continuous temperature ramps • Temperature measured directly in sample OPTICS-BASED, MULTIWAVELENGTH CALIBRATION • For CD accuracy (Chirascan V100) CHIRASCAN CONTROL • Easily define run parameters and store routine protocols • Saves time with scheduled start-up/ shutdown of lamp and N2 supply • Fail-safe lamp switch-off if N2 flow drops • Ensures O2-free conditions with N2 purge GLOBAL THERMODYNAMIC ANALYSIS • Derive melting points and enthalpies from multiwavelength, thermal denaturation...

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-6

OPTIMIZE SAMPLE CONCENTRATION AND ABSORBANCE: CUVETTES AND HOLDERS Selecting a suitable cuvette with optimal pathlength is critical to acquisition of highest quality data. Cuvettes for Chirascan systems are manufactured from far-UV quartz to enable analysis of secondary structure. The range of cuvettes and compatible holders provides full flexibility when optimizing sample concentration and absorbance. For secondary structure (far-UV) analysis 0.5 mm 175 μL one-piece stoppered cuvette Adaptor for 0.5 mm and 1 mm one-piece cuvettes Supplied with Chirascan and Chirascan V100 Not suitable for fluorescence...

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CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS-7

EXPAND CAPABILITIES WITH DEDICATED CHIRASCAN ACCESSORIES CCD FLUOROMETER Monitor changes in fluorescence Increase capacity and productivity Monitor concentration- and pH-dependent changes in CD, fluorescence or absorbance Use linear dichroism to gain insight into conformation and orientation of molecular structures INTEGRATING SPHERE AND SOLID SAMPLE HOLDER Characterize fast reactions, complement CD spectra with kinetic information See changes in chirality of solid state samples ALSO AVAILABLE A fluorescence anisotropy detector to characterize ligand binding events. An optical rotatory despersion...

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