CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS
7Pages

CHIRASCAN AND CHIRASCAN V100 VERSATILE RESEARCH SYSTEMS HIGH PERFORMANCE, READY TO RUN • Determine structural and thermodynamic properties - Gain insight and detect changes in secondary and tertiary structure - Determine response to thermal or chemical changes - Study folding and unfolding mechanisms • Achieve highest sensitivity and accuracy • Generate highest quality data • Optimize sample concentration and absorbance • Expand capabilities with dedicated Chirascan accessories Discover when change is sign

DETERMINE STRUCTURAL AND THERMODYNAMIC PROPERTIES Gain insight and detect changes in secondary and tertiary structure Secondary structure: far-UV spectrum of a globular protein Tertiary structure: near-UV spectra of two monoclonal antibodies combined contributions from a-helix and β-sheet Peak positions Circular dichroism / mdeg. Circular dichroism / mdeg. Simultaneous acquisition of CD and absorbance spectra, 0.5 mm pathlength, Chirascan V100. Courtesy of leading research university, Germany Differences between near-UV spectra due to slight changes in orientation of aromatic moieties,...

ACHIEVE HIGHEST SENSITIVITY AND ACCURACY Since their introduction in 2005, Chirascan™ systems have continued to feature in thousands of peer-reviewed publications covering a wide range of research areas. Chirascan V100 now offers the increased sensitivity and accuracy preferred for CD analysis of biomolecules. • Avalanche photodiode detector enhances sensitivity • Increased signal:noise compared to conventional photomultiplier • Accurate normalization from simultaneous measurement of absorbance and CD Increased sensitivity when sample is limited Increased sensitivity: faster measurements...

READY TO RUN – GENERATE HIGHEST QUALITY DATA Chirascan systems are supplied with features and accessories required for acquisition of high quality CD data – from built-in temperature control during analysis to cuvettes for the most common analytical conditions.* A basic training program follows installation to familiarize users new to Chirascan. AVALANCHE PHOTODIODE DETECTOR (CHIRASCAN V100) • Highest sensitivity (high signal: noise) PHOTOELASTIC MODULATOR PHOTOMULTIPLIER DETECTOR (CHIRASCAN) • Converts horizontally polarized light to circularly polarized light. Alternates between left- and...

CONTROL AND ANALYSIS SOFTWARE TEMPERATURE-CONTROLLED SAMPLE CHAMBER • Consistent analytical conditions • Continuous temperature ramps • Temperature measured directly in sample OPTICS-BASED, MULTIWAVELENGTH CALIBRATION • For CD accuracy (Chirascan V100) CHIRASCAN CONTROL • Easily define run parameters and store routine protocols • Saves time with scheduled start-up/ shutdown of lamp and N2 supply • Fail-safe lamp switch-off if N2 flow drops • Ensures O2-free conditions with N2 purge GLOBAL THERMODYNAMIC ANALYSIS • Derive melting points and enthalpies from multiwavelength, thermal...

OPTIMIZE SAMPLE CONCENTRATION AND ABSORBANCE: CUVETTES AND HOLDERS Selecting a suitable cuvette with optimal pathlength is critical to acquisition of highest quality data. Cuvettes for Chirascan systems are manufactured from far-UV quartz to enable analysis of secondary structure. The range of cuvettes and compatible holders provides full flexibility when optimizing sample concentration and absorbance. For secondary structure (far-UV) analysis 0.5 mm 175 μL one-piece stoppered cuvette Adaptor for 0.5 mm and 1 mm one-piece cuvettes Supplied with Chirascan and Chirascan V100 Not suitable for...

EXPAND CAPABILITIES WITH DEDICATED CHIRASCAN ACCESSORIES CCD FLUOROMETER Monitor changes in fluorescence Increase capacity and productivity Monitor concentration- and pH-dependent changes in CD, fluorescence or absorbance Use linear dichroism to gain insight into conformation and orientation of molecular structures INTEGRATING SPHERE AND SOLID SAMPLE HOLDER Characterize fast reactions, complement CD spectra with kinetic information See changes in chirality of solid state samples ALSO AVAILABLE A fluorescence anisotropy detector to characterize ligand binding events. An optical rotatory...