GenASIs HyperSpectral
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GenASIs HyperSpectral HyperSpectral Imaging Solutions for Biomedical Applications

GenASIs Platforms Designed to Meet the Requirements of Any Laboratory - Large and Small ASI’s scalable and modular platforms are designed to meet current and future laboratory needs. As your caseload increases, so can your lab enabling growth from a single slide stations to 9-slide or 81-slide scanning stations, additional workstations, dedicated servers, and modular LIS/LIMS connection to automate your workflow. GenASIs 7 enables processing of more cases, quicker, with better clinical results. GenASIs Capture & Analysis platform is a high-end computer aided diagnostic system with multiple...

GenASIs HyperSpectral Insights Into the Molecular and Structural Content of Images Spectral imaging represents an enabling technology for a spectrum of applications in biomedical imaging and remote sensing. In all of the applications, the foundation technology remains virtually identical, thereby providing tremendous technology leverage. Spectral Imaging describes image acquisition and analysis method which combines spectroscopy, multi-dimensional imaging and computing to delineate the way light reacts with a sample in order to quantify and analyze information that might otherwise be...

GenASIs HyperSpectral • Insights Into the Molecular and Structural Content of Images The unique design of GenASIs HyperSpectral platform with SpectraView allows the simultaneous processing of the entire input image without the need for sequential spatial or wavelength scanning of the sample or the sample illumination. The core of the HyperSpectral platform is a common path Sagnac interferometer, mounted on a rotatable disk, to which a CCD camera is coupled. The light beam which enters the triangular interferometer, is split into two beams (a transmitted and a reflected beam), which travel...

GenASIs SpectraView Capabilities Addressing the Needs in Microscopy and Cell Biology Differentiate Uncover chemically similar areas hidden to the eye. Create color-coded maps of chemical similarities and differences. Compare the chemical makeup of components between different images. Choose a region with the qualities you want to analyze, GenASIs SpectraView automatically finds all the other similar regions in the image Original Image Classified Image Separate Separate spectral components to view them as individual image layers. Automatically detect objects and classify them based on...

GenASIs SpectraView Specifications Addressing the Needs in Microscopy and Cell Biology • Spectral range: 400-1000 nm, covering the entire visual range and the low NIR range • User defined spectral resolution is 6 nm at 400 nm, with capability for improved spectral resolution up to 3nm with the high resolution setup option • Dual mode enables both hyperspectral capture and regular monochromatic imaging on the same system • The system maintains the polarization of the measured light • Transmission efficiency is higher than 80% for the entire visual spectral range, making GenASIs SpectraView...

GenASIs SpectraView for Spectral Karyotyping Spectral Karyotyping - SKY Utilizing its high spectral resolution, the GenASIs HyperSpectral platform detects and identifies a plurality of colors simultaneously. Spectral Karyotyping (SKY) is an example, in which each of the 24 chromosomes is identified based on a unique color combination. The GenASIs HyperSpectral System HiSKY is widely used in cancer research and in pre- and post-natal analysis. Color Banding N. Kakazu, T.Abe (2006) Multicolor banding technique, spectral color banding (SCAN): new development and applications. Cytogenetic and...

GenASIs SpectraView for Pathology Spectral Un-mixing with Fluorescence and Bright Field for Cell Identification The detailed spectral information enables performing accurate spectral un-mixing, extracting a set of images corresponding to the pure colors from which an image is constructed. The physical meaning of this operation varies between fluorescence and brightfield. In fluorescence each un-mixed pure image corresponds to any of the following: • A single dye that is included in the sample Unwanted background that is caused either by residual of specific dyes or by auto-fluorescence As a...

GenASIs SpectraView for Pathology Spectral Un-mixing in Bright Field and Cell Identification Pap-Smear Analysis Quantitative analysis of Pap-stained slides. The image of the smeared cells is measured in bright field. Each component of the Pap-stained slide has a different spectrum. These spectra are used to identify the different entities. SUN algorithm is used for the analysis. Chronic Lymphatic Leukemia (CLL) Normal and CLL cells have different spectral signatures. These spectra were used to classify the images on the right (classified colors) Z. Malik et al, Spectral morphometric...

GenASIs SpectraView for Cell Biology Multiplexing Colors for Parallel Analysis Intracellular Fate of Liposomes Multicolor study with five dyes and COS-7 cells: (A) original view, (BI) brightfield image of the same group of cells, (BII-VI) spectrally decomposed single-color images: (II) LysoTracker Red, (III) FITC-dextran, (IV) Rh-PE, (V) Vybrant DiO, and (VI) DAPI. Ulrich Huth, et al. Fourier Transformed Spectral Bio-Imaging for Studying the Intracellular fate of Liposomes Cytometry Part A 57A:10-21 (2004) Stem Cell Identification Multicolor immunofluorescence of human mesenchymal stem...

GenASIs SpectraView for Biomedical Research Catering to General Research Needs Polystyrene Beads 5 nm beads, coded to 10 classes by fluorescent dyes (combinatorial stained) are spread over a slide or titerplate membrane. To each group of beads a specific Single Nucleotide Polymorphisms (SNP) is attached. The detection of the SNPs is done by a fluorescent reporter molecule that emits in a different spectral range than the color beads. Beads are imaged with GenASIs SpectraView and classified based on their spectral signature. The attachment of SNPs to the coded beads is detected by the...