Simply and Reliably Size DMPK Repeat Expansions within a Single Day The AmplideX® PCR/CE DMPK Kit* introduces a groundbreaking alternative for the detection and sizing of CTG repeats within the DMPK gene. Challenged by very large trinucleotide expansions, DMPK analysis has required the use of Southern blot technology, thereby limiting many laboratories’ ability to resolve these repeat sequences. By introducing a simple, streamlined, PCR-only workflow, the AmplideX PCR/CE DMPK Kit* can replace Southern blot and put DMPK analysis within the reach of laboratories everywhere. REDUCED COMPLEXITY OPTIMIZED WORKFLOW • Proprietary PCR solutions for GC-rich amplification and detection • May eliminate need for Southern blot • Resolves zygosity and detects mosaicism • Direct injection of PCR products into CE instruments • Fully kitted, end-to-end solutions that significantly reduce hands-on-time • Sample-to-result possible within a single shift QUALITY PERFORMANCE • Accurate sizing of alleles ≤200 CTG repeats and detection of all alleles >200 repeats using CE • Optional AGE protocol allows sizing of alleles up to 1000 repeats • Analysis tool converts raw base pair data into repeat number Figure 1. AmplideX PCR/CE DMPK Workflow, including optional AGE workflow fo

Figure 2. Comparison of residual clinical sample results between Asuragen (AmplideX Kit) and partner lab (PCR + Southern blot). Figure 3. Sensitive and accurate sizing of DMPK expansions up to 200 repeats. Figure 4. Clear resolution of zygosity. KIT ORDERING INFORMATION AmplideX PCR/CE DMPK Kit* *For Research Use Only. Not for use in Diagnostic procedures. Asuragen, Inc. 2150 Woodward Street, Suite 100 Austin TX 78744 asuragen.com 2000-027 Rev B 08/2018