Sensitive and Accurate Sizing of CAG Repeats in the HTT Gene The AmplideX® PCR/CE HTT Kit makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. Accurate sizing of the CAG expansions in HTT is challenged by the presence of adjacent SNPs and variable CCG repeats, which can complicate primer binding and cause allele dropouts. With its unique, two-primer design, the AmplideX PCR/CE HTT Kit overcomes these challenges to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA. REDUCED COMPLEXITY OPTIMIZED WORKFLOW • Proprietary PCR solutions for GC-rich amplification and detection • Eliminates need for multiple PCRs – one result, straightforward analysis • Resolves zygosity and detects large expansions • Fully kitted, end-to-end solutions that significantly reduce hands-on-time • Sample-to-result possible within a single shift • Identical PCR and CE conditions as the AmplideX® PCR/CE DMPK Kit QUALITY PERFORMANCE • Reliable, unambiguous results and a robust stutter peak pattern • Accurate sizing across the entire CAG repeat range • Software converts raw base pair data into number of repeats Figure 1. AmplideX PCR/C

Figure 2. Repeat profile is conserved across known SNPs *Additional minor peak consistently identified with Asuragen assay Figure 3. 100% Concordance (±1 repeat) observed with previously characterized samples, as reported by Kalman et al. (2007) Figure 4. Clear resolution of zygosity. KIT ORDERING INFORMATION AmplideX PCR/CE HTT Kit* *For Research Use Only. Not for use in Diagnostic procedures. Asuragen, Inc. 2150 Woodward Street, Suite 100 Austin TX 78744 asuragen.com 2000-064 Rev A 12/2018