COOL SOLUTIONS PELTIER “My lab has been using the Cool Solutions device for over ten years, and it has been a fantastic unit for characterizing the properties of thermosensitive TRP channels, both native and cloned. Its Peltier cooling and heating provide accurate and quiet thermal stimuli in our electrophysiology and imaging experiments, with superior range, speed, and reliability.” Your thermoTRP channels won’t know what hit them. Dr. David Julius Department of Physiology University of California, San Francisco Cool Solutions CS-1 Peltier Thermal Stimulus Delivery System • Heat from 20 to 50˚C in 3 seconds, then cool to 2˚C in just a few more seconds — all controlled by your electrophysiology software The main controller is ready for 19" rack mounting (size 2U). 483mm w x 90mm h x 395mm deep 19" w x 3.5" tall x 15.5" deep 6.5 kg = 14 lbs. • Display Peltier, heatsink or bath temperature • Set the desired temperature from the front panel or from your computer: The CS-1 can generate any desired timecourse of temperature change, by simply converting a voltage from your interface into a temperature • Optically isolated bath sensor for low noise • Suitable for single channel (no detectable noise added), whole cell patch clamping, or fluorescent ion imaging of groups of cells • Optional water cooling for low-temperature work • Two output BNCs to monitor and record two temperatures externally (e.g. Peltier and bath) Three externally amplified • All components included - five minute setup temperature sensors including an • Tried and tested design, used in major thermoTRP labs for over a decade (see list of publications) optically isolated bath sensor, for vir

12V batteries and charger included for noise-free heating and cooling This temperature controller is for superfusing small areas (e.g. groups of cells) in a recording bath, at a precisely controlled and rapidly adjustable local temperature. The system has been especially developed for patch clamp experiments in order to apply thermal stimuli without introducing noise or interference. It is also very suitable for intracellular ion imaging in cultured cells or tissue slices, oocyte recording, intracellular microelectrode recording or extracellular (e.g. brain slice) recording. o » Special 0TC error...