PERFUSION SYSTEMS
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PERFUSION SYSTEMS - 1

PERFUSION SYSTEMS “­ am writing to tell you I ­ how pleased I am with the ValveBank8 Perfusion System. It’s great that I can load the reservoirs, press go, and start recording. I can read papers rather than fussing with solutions and switching valves every 10-15 minutes. Since solutions are switched at exactly the same time from one experiment to the next, I have been able to automate my data analysis protocols as well.” Increase reproducibility with fewer hours in lab. Brain Slice Field Recording Unattended solution delivery using a ValveBank8 AutoPrime Perfusion System Dr. M. Bruce MacIver, M.Sc., Ph.D. Department of Anesthesia Stanford University Medical Center ­­­ Gravity Perfusion Systems ­ • Unattended solution switching Avoid vibrations from switching valves by hand. The ValveBank®or ValveLink® controller handles all solution delivery so you can watch results – not switch stopcocks. Many special features are included for easy perfusion control. Low noise circuitry. • Increased reproducibility Valve switching is accurate to 0.01 seconds with programs up to 99 hours long under microprocessor control. Consistent liquid delivery means better data. • Pinch, PTFE-Inert and Lee™ Valves Choose between speed, cost, and ease of cleaning. Several options are available for fittings and reservoirs. • Manual and external valve control Flexible design. Easy cleaning and calibration. Slave mode valve operation controlled by your computer, pClamp, Pulse, Acquire,

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Valve Choices: Pinch Valves for Reduced Maintenance Easiest valves to clean and switch tubing. Liquids never touch the valves. Switches in 30-50 ms. 1/32” i.d. silicone tube passes through, and is pinched closed by solenoid activation. All AutoMate Scientific valves include an individual indicator LED. Our new aluminum enclosure keeps the valves dry from spills and offers luer lock ports for syringe reservoirs. • PTFE-Inert Valves for Fast Switching Required for fast kinetics applications. Excellent chemical and corrosion resistance. Non-stick surface resists particles and chemical...

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Luer-lock Fittings in PTFE-lnert Valves m Luer-lock fittings in PTFE-Inert valves allow direct connection of syringe reservoirs for minimal dead volume. PTFE-Inert Valve Fitting Choices Hose Barb M' • Standard • Available for 1/8" and 1/16" i.d. soft tubing Lure-Lock • For direct attachment of syringes • Eliminates extra tubing between reservoirs and valves • Includes 2-way stopcocks and 35 ml syringes Nut & Ferrule P • HPLC-like, screw-in fittings for rigid, small-diameter ( 1/16" o.d.) tubing C€ AutoMate Scientific* READY FOR RESEARCH.™ Perfusion Systems Ordering Information Part No....

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Perfusion System How to configure and order a perfusion system. Valve Controllers AutoMate Scientific sells complete perfusion systems as well as individual components. This can make it confusing for customers to determine which pieces they need to order. We hope this guide helps. All of the items mentioned below are explained in more detail on the following pages, or you can quickly customize a complete perfusion system on our web site at http://www.autom8.com/build_your_own.html. 1. Gravity, bath perfusion systems 2. Pressurized, fast, local drug application 3. Temperature control 4....

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ValveLink controller is designed for customers who have digital/TTL outputs from their computer -- from data acquisition software like pCLAMP, Pulse, Patchmaster, WinWCP, MetaMorph, etc. or I/O hardware with software like LabView or MATLAB. Alternatively, our ValveBank controller is completely programmable without needing a computer. It is ideal for setups without computer control or imaging software with only 1-2 digital trigger or shutter outputs. 2. Pressurized, fast, local drug application For customers needing faster switching times (ligand-gated channels, etc.) we recommend local drug...

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QuickStage Chamber AutoMate Scientific’s standard perfusion systems are all room-temperature by default, but we offer several temperature control options: 1) ThermoClamp® inline heater, 2) BubbleStop® syringe reservoir heater, 3) QuickStage™ stage heaters, 4) Cool Solutions peltier heater/cooler, and 5) objective heaters. Most of the thermal mass your cells experience originates from the liquids flowing over them. Therefore, the most effective temperature control is a good inline solution heater (or cooler) which is positioned as close to the prep as possible. Our ThermoClamp-1 maintains...

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Perfusion Packages To order a pressurized or temperature-controlled perfusion system, you should start with one of our unheated gravity systems, and add the additional pieces you need. Here are some examples: Example Simple Systems Ordering Information 13-11-57 ValveBank8 Lee Valve Perfusion System 01-05 Low-noise grounding Brain Slice Chambers Example Advanced Systems Ordering Information Part No. S-BSC1 Brain Slice Chamber-1: Interface and Submerged We offer a great web form to help build you own perfusion system. Just answer a few questions, enter your contact info, and click Submit...

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