NAHITAthermal cyclers figure1 : PCRdiagram Thanks to their highest features and incredible price, Nahita thermal cyclers are the first choice in a wide range of laboratories and research centres to perform the polymerase chain reaction or PCR, technique that allows obtaining millions of copies of a certain DNA template. The most common protocol of PCR consists of a series of 20-30 cycles, in each one of them the new copies of DNA are multiplied exponentially. Each cycle is divided into 3 steps at different temperatures: Denaturation: separation of the two DNA strands by submitting the sample...

NAHITAthermal cyclers general features Nahita thermal cyclers are equipped with Peltier-based technology that allows heating and cooling sample tubes by simply reversing the electric Heated and adjustable lid current, and that, together with the use of aluminium alloy blocks, allows obtaining the maximum performance and accuracy of the PCR technique: High quality and durability of Peltier-based components increasing the life time of the equipment Compact, light and noiseless design; for space-saving and easy setup and transportation LCD display that offers complete information about the different...

NAHITAthermal cyclers Made of aluminium alloy to reach the maximum performance and accuracy of the tech- The features of the lid of Nahita thermal cyclers increase accuracy and reproducibility of nique, it presents a combined configuration for different type of tubes avoiding the ne- cessity of interchanging blocks and minimizing breakage and problems as well as saving space and money. Maximum capacity according to the tube used: 96 x 0.2 mL standard tubes 77 x 0.5 mL thin-wall tubes 8 x 12 tube-strips of 0.2 mL 12 x 8 tube-strips of 0.2 mL 1 x 96 wells plate Heated lid up to 105 ºC to avoid...

NAHITAthermal cyclers [Application software] 11 Memory for more than 125 different programs 21 2 different temperature control modes available: ■ Block temperature: the programmed temperature is that of the block I Tube temperature: by inputting the volume of sample in tubes the estimated tem- perature inside the tubes can be programmed 31 Possibility of performing special variants of the PCR technique since for each step of a cycle the following parameters can be programmed: ■ Temperature increase/decrease: in each cycle the step temperature can be incre- ased or decreased respecting to the...

NAHITAthermal cyclers [Gradient function (only model MG96G)] Length and temperature of each step, especially annealing step, must be determined for each PCR reaction. Gradient function, available in Nahita thermal cycler [Temperature gradient] model MG96G, allows programming a gradient up to 30 ºC so as a different temperature is set in each column of the block; this way, different conditions can be tested in a single experiment speeding up and making easier the set-up of the technique.

NAHITAthermal cyclers technical features Code Model Technology Block Capacity Compatibility Temperature Max. heating rate Max. cooling rate Temp. uniformity Temp. accuracy Temp. Control mode. Adjustable heating rate Display Programming Programs Max. number cycles Temp. increase/decrease Time increase/decrease Auto pause Auto restart Hold at 4 ºC Running time display Lid Temperature Height Auto off Gradient Gradient range Accuracy Column uniformity Temp. range. Dimensions approx. (LxWxH) Weight approx. Power 96 x 0.2 mL; 77 x 0.5 mL Tubes of 0.2 mL and 0.5 mL (thin-wall) 8 and 12 tube-strips 96...

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