Automated Organoid Counting CytoSMARTTM Software Package Accurate Organoid Counting in a Flash Together with Corning®, CytoSMART already introduced an automated cell counter for counting of mammalian cells called the Corning® Cell Counter. Now a new, powerful and simple software application has been developed that enables organoid counting with the Corning® Cell Counter (fig 1). Manually counting organoids is a routine part of laboratory operations, but is time consuming and user dependent. To overcome these issues CytoSMART has introduced an organoid counting application that is: Fast & Accurate - thanks to its cloud-based image analysis algorithm Low-cost - works with common reusable glass Figure 2: Software interface. The output of the analysis provides the user with organoid size and count. The results are updated in real time based on user defined size gating. hemocytometer. No consumables required Precise - software allows for data gating and image Organoid Counting in 3 Seconds selection for statistical analysis The Organoid Counting software can analyze a single image in less than three seconds* utilizing the CytoSMART™ Cloud. This cloud computing ability enables the rapid images analysis. High Accuracy Figure 1: Detecting Organoids using the CytoSMART Organoid Counting Software. Brightfield images of organoids are analyzed by the image analysis algorithm and allows for organoid detection over a wide range of sizes. Easy to Use The Automated Organoid Counting software is easy to use: simply connect the Corning® Cell Counter to your computer or tablet and start the application for Organoid Counting. Place the loaded counting chamber on the stage. Focus on your organoids and press the Count button. The Organoid Counting application has an intuitive interface, which allows user specific size gating and image selection (fig 2). The software contains an image analysis algorithm that optimized for organoid detection. This state-ofthe-art analysis tool allows for optimal accuracy in data acquisition. Users obtain information on the quantity and size of the organoids in their sample. This data is displayed in separate interfaces that provide a clear overview of the characteristics of the organoid population: Single images - can be inspected one-by-one (fig 2) Organoid size distribution - a histogram of the organoid sizes is provided to get an overview of the size distribution in your sample (fig 3) Average sample concentration - is provided based on images selected by the user. The count avarage is displayed in a bar chart next to the counts from single selected images to inspect the uniformity of your data (fig 4). * Measured using a 73 Mbps download speed and a 20 Mbps upload speed. Actual speed can vary depending on the internet connecti

Low Cost Like Manual Counting The Organoid Counting software runs on the Corning® Cell Counter. Similar to counting mammalian cells users can use an hemocytometer or organoid proof counting slides to image their samples. Users have the possibility to use re-usable counting slides, to enjoy the benefits of automated counting without the cost of disposable slides. Accessible Data Anywhere, Anytime Figure 3: Get an overview of the size and number of the organoids in your sample. Select the images you want to analyze on the left side of the interface. The software automatically calculates the average...