CytoSMART Exact FL fluorescence cell counter
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Fluorescence cell counter with the best eye for detail CytoSMART™ Exact FL

Accelerate your research with fluorescence cell counting Automated fluorescence cell counting has transformed the process of cell quantification, as it offers a great deal of flexibility and accuracy, and can be used when bright-field image analysis is not possible. The CytoSMART Exact FL is an automated, dual fluorescence cell counter with an expanded field of view and unmatched resolution and magnification power. Using an advanced optical system and AI-powered image analysis software, the CytoSMART Exact FL calculates the exact number of cells in a sample and provides reliable assessment...

The use of fluorescence cell counting significantly accelerates experimental work in the fields of cancer biology, immunology, tissue engineering, and cell therapy. Numerous cell-based assays significantly benefit from fluorescence cell counting due to the number of issues associated with detecting and analyzing mammalian cells using colorimetric dyes (e.g. Trypan Blue). The CytoSMART Exact FL, fitted with red and green fluorescent channels, in combination with appropriate fluorescent dyes, can assist you in examining the following features (Fig. 1): + + + + + + + Cell viability Small and...

State-of-the-art optics AI-powered algorithms Using the 6.4 MP CMOS camera combined with 10× magnification, the CytoSMART Exact FL can visualize and count cells down to 4 µm in diameter. With increased field of view and multicount feature, it is also possible to take multiple snapshots of the same sample at various slide positions, which increases the accuracy and reproducibility of a cell count. Manual cell counting introduces userto-user variability to a scientific study. In addition, the process of manual cell counting is challenging and timeconsuming, especially when working with cells...

Automated software Innovative organoid updates & secure cloud counting software data storage All CytoSMART devices are connected to the CytoSMART cloud environment. This means that the data generated by the Exact FL fluorescence cell counter is processed, analysed and stored in one accessible and secure place, protected by a multi-layered cyber security system. Novel software updates are also introduced via the CytoSMART Cloud. With only a few clicks our customers can download and install these add-ons to make their user experience even more seamless. Importantly, the added features will...

Frequently Asked Questions Q: Which fluorescent dyes can be used with the CytoSMART Exact FL? A: Many different fluorescent dyes can be used, as long as the fluorescent dye’s excitation and emission spectra correspond with the fluorescent filters of the Exact FL (green – excitation: 452/45 nm, emission: 512/23 nm; red – excitation: 561/14 nm, emission: 630/90 nm). Some examples are propidium iodide (PI) and red fluorescent protein (RFP) for the red channel, and acridine orange (AO), calcein-AM, and green fluorescent protein (GFP) for the green channel. Q: Is it possible to control the...

Technical Specifications Size range - cells 4 - 70 |jm Size range - organoids 10 |jm to 100/200 |jm* Loading volume (1 chamber) 10 |jL Field of view 1 count: 2.1 mm2 8 counts (multi-count): 16.8 mm2 Autofocus Yes Channels Bright-field, green and red fluorescence channels Fluorescence filters Green - excitation: 452/45 nm; emission: 512/23 nm Red - excitation: 561/14 nm; emission: 630/90 nm Magnification 10X (digital zoom 20X) Image resolution 2072 X 2072 pixels Data storage The CytoSMART Cloud Computer requirements Windows 10, USB 3.0 Counting chamber Reusable (glass) or disposable...

About CytoSMART CytoSMART Technologies is a specialist in development and manufacting of smart microscope systems for life science labs. The company was founded in 2012 by a team of biologists and engineers who were convinced that a new generation of miniaturized microscopes, powered by artificial intelligence for image analysis, would allow biologists to make discoveries more efficiently and at a larger scale. In 2018 CytoSMART was selected by Microsoft for its prestigious Scale Up program. CytoSMART’s microscopy solutions are used in over a thousand laboratories around the world....

CytoSMART™ Technologies B.V. Emmasingel 33 Website: www.cytosmart.com For Research Use Only. Not to use in diagnostics procedures. ©2021 CytoSMART™ Technologies B.V., the CytoSMART™ logo is a trademark and/or registered trademark of CytoSMART™ Technologie