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CytoSMART Omni: Label Free Assay - Colony Formation

CytoSMART Omni: Label Free Assay - Colony Formation

Product catalog summary
Overview: The document discusses the use of time-lapse brightfield imaging to study various aspects of cell behavior, including health, viability, colony formation, and migration. The CytoSMART Omni device is highlighted as a tool for performing kinetic assays that capture cellular behavior over extended periods.
Colony Formation: Colony formation assays are used to assess the clonogenicity of cells. The CytoSMART Omni captures bright-field images within cell culture incubators over several days, providing a comprehensive overview of whole-well surfaces. Integrated image analysis algorithms in the CytoSMART Cloud analyze the number of colonies, their circularity, and size. The system allows for easy extraction of both numerical data and images post-analysis.
Automated Colony Detection: The document describes the automated detection of colonies using HEP-G2 cells with a colony mask overlay. The image detection algorithm filters colonies based on user-defined specifications such as size, circularity, and perimeter, tracking their growth over time. An example is provided where cells were recorded for 75 hours with a 3-hour time interval.
Data Visualization: Graphs included in the document illustrate the changes in colony size, count, and circularity over time, providing visual insights into the growth dynamics of the colonies.
Usage Note: The CytoSMART Omni is intended for research use only and is not suitable for diagnostic purposes.
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Catalog excerpts

CytoSMART Omni: Label Free Assay - Colony Formation-1

CytoSMART Omni live-cell imaging Time-lapse brightfield imaging to study cell health, viability, colony formation and migration Users can perform kinetic assays that capture cellular behavior for days or even weeks at a time. cell level detail full plate overview Automated colony detection: HEP-G2 cells with colony mask overlay. The image detection algorithm filters colonies based on user specifications on size, circularity and perimeter and follows their growth over time. Cells were recorded for 75 hours with a 3 hour time interval. 0,8 Colony formation assays provide insight into 6E+03 5E+03 0,6 the clonogenicity of cells. The formation and 4E+03 0,4 growth of colonies can be monitored using 3E+03 2E+03 0,2 the CytoSMART Omni. Bright-field images 1E+03 0E+00 are automatically generated from within 0,0 cell 0 0 20 40 60 80 culture incubators over periods of days. Whole�me [hours] well surfaces are captured to get a full sample 9E+04 6E+03 overview. 8E+04 stdv colony size [μm²] Colony count Integrated image analysis algorithms in the 3E+03 CytoSMART Cloud provide information on the 2E+03 1E+03 number of colonies, colony circularity and size. 0E+00 The highlighted orange areas in the image 0 0 20 40 60 80 indicate the colonies that are used for the �me [hours] analysis. Both the numerical data and images can easily be extracted after analysis.* 4E+03 https://www.cytosmart.com/products/omni *Research use only. Not intended for diagnostic purposes

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.