Fluorescence cell counter with the best eye for detail The Exact FL is a compact and versatile automated fluorescence cell counter that provides highly accurate cell counts due to its high-resolution camera, high magnification and large field-of-view combined with sophisticated AI-based image analysis. Whether you need to count primary cells, heterogeneous samples, small cells, such as PBMCs or need to determine the viability or transfection efficiency of your samples, the CytoSMART Exact FL can do it all due to its eye for detail. Benefits of fluorescent cell counting Many labs still count cells manually, which is subjective and time- Due to its high-quality optics and AI-based image analysis, the consuming. Even for the trained eye, it can be challenging to CytoSMART Exact FL is one of the most detailed and versatile distinguish cells from cell debris. Next to this, the commonly used fluorescence cell counters available. It not only provides the Trypan Blue stain to distinguish dead from live cells, does not option to count cells using both brightfield and dual fluorescence always stain the cells properly. This makes it hard to determine (red and green), but also enables analysis of viability, transfection whether a cell is dead or alive. Furthermore, countless hours efficiency and many more applications. spend behind the microscope to count hundreds of cells can be very labour-intensive. Cell viability sample 4 Tutorial Brightfield automated cell counters drastically decrease the time needed for cell counting and the integrated image analysis Cell size algorithms provide objective cell counts. However, not all issues related to distinguishing cells from debris and live from dead cells are resolved. To solve this, fluorescent cell counting provides a solution. By using fluorescent dyes, it is very easy to distinguish cells from debris, since cellular debris is not stained with the fluorescent dyes. The commonly used red dye Propidium Iodide (PI) only binds to DNA of cells with an impaired cell membrane (i.e. dead cells) making it more reliable than Trypan Blue. PI is often 8 Exclude (empty) Dilution used in combination with Acridine Orange (AO), a green, cellpermeable, DNA-binding dye that stains all cells in the sample. https://cytosmart.com/products/cytosmart-exact-fl *Research use only. Not intended for diagnostic purposes. Figure 1. The multicount feature allows users to take images at different positions of the same sample, providing an average and standard deviation to increase accuracy and precision.

The CytoSMART Exact FL provides High quality optics for detailed analysis: The 6.4 MP CMOS camera Concentration range: 5·104 - 1·107 cells/ml combined with 10x magnification allow the user to count cells as small as 4 µm with high accuracy, providing detailed images of your count for review. Size range organoids: 10 – 200 µm Measurement time: < 3 sec. (depends on Multi-count feature: By taking up to eight images of the same internet connection) sample, the counted area increases up to 17 µm (fig. 1). This Sample volume: 10 µl (20 µl for organoids) results in an increased accuracy and precision...