Azure™ cSeries A new way to see the light.

Infrared Laser Excitation for Quantitative Western Blot Imaging in the NIR Improve Your Data Quality Imaging with infrared dyes offers signal stability and low background fluorescence. Additionally, imaging with NIR dyes allows you to study multiple proteins in a blot, even if those proteins overlap in molecular weight. Azure Biosystem’s cSeries laser technology offers two IR detection channels enabling a user to image more than one protein in an assay. Sensitive Detection of Proteins with Chemiluminescent Westerns Get Out of the Dark The Azure cSeries provides accurate and fast chemiluminescent...

Azure cSeries Features Easy Installation Just unpack, power-up and start. No technician required. Compact, Integrated Design A unique design and integrated tablet computer gives this system a small footprint, saving you room for other tools on the benchtop. Simple Image Saving Save to a USB drive, to a network via WiFi or Ethernet, or print to a printer. 10.1” tablet controls the system with the touch of a finger. Networking is easy, with WiFi or Bluetooth. Laser Diodes at 660 nm and 785 nm Narrow band excitation enables NIR applications with better sensitivity than LEDs or white light sources....

High Resolution Camera Capture the finest details of your sample. Deep Peltier Cooling -50°C regulated cooling for low-noise images. Dual-Focus Technology Image samples in perfect focus, and with optimal lens settings, without having to touch the camera. 7 Position Filter Wheel The motorized filter wheel allows flexibility of applications. Chemi Blot Shelf Place chemiluminescent blots closer to the detector on an adjustable shelf (stored in the door when not in use). Connect to a drive or network, or attach a thermal printer. The large field of view allows imaging of large gels and blots, or...

Quantitative Analysis of Infrared Fluorescent Westerns Why Fluorescent Westerns? Detect Multiple Proteins in One Assay Two infrared fluorescent channels for detection enable simultaneous two-color analysis. Save time and sample by detecting multiple proteins in the same assay without needing to strip and reprobe the blot, or having to run a second blot. Multichannel fluorescent detection also enables normalization of your intensity values to a loading control to correct for inaccuracies in loading. No Rushing to the Darkroom Time Elapsed With infrared dyes, data are not contingent on the lifespan...

Why IR Fluorescent Westerns? Increased Sensitivity Because of Reduced Membrane Autofluorescence at Infrared Compared to Visible Wavelengths 2000 Median Pixel Intensity Nitrocellulose membranes were imaged using lasers with wavelengths at 660 nm and 785 nm. The same membranes were scanned for the same time at 525 nm and 630 nm wavelengths. Autofluorescence was much lower when using infrared wavelengths. Why Choose the Azure c500 and c600 for IR Fluorescent Westerns? Azure cSeries Competitor Azure cSeries Competitor Relative Absorbence Other CCD based imaging systems use LEDs for excitation of...

Western Blotting with Chemiluminescence No Need to Change Your Protocol Already using an HRP substrate? No matter what substrate you are using, the Azure cSeries systems are compatible with your current protocol. Instead of using film and a developer, simply place your blot in the cSeries to get great results. No Need for a Darkroom Get the sensitivity you trust from film, with the convenience of digital imaging. Using the highresolution, deeply cooled camera of the Azure c300, 400, 500 or 600, and the 0.95 fast lens technology, you can capture images with the same sensitivity as film. The system...

16 bit Imaging for a Wide Dynamic Range A Western blot was imaged on both the Azure c600 (a 16 bit system) and a competitive 12 bit system. second exposure appears similar system produces High Resolution Provides Faster Chemi Acquisition A CCD camera has the ability to combine multiple pixels into a single larger pixel or "super pixel." This is a technique known as binning. Binning of 1X1, means the full resolution of the camera is used to capture an image. A binning of 2X2 means that the areas of 4 adjacent pixels are combined into one larger pixel, and so on. In a 2X2 bin, the sensitivity to...

Flexibility for Your Applications A Total Solution for Western Blot Imaging and Gel Documentation The cSeries is a multichannel, multimodal imager, with IR, visible light, and UV excitation channels. Detect Cy dyes, Alexa dyes, Safe dyes, and more. Light Sources The flexibility of Azure’s cSeries comes from the wide variety of light sources. Visible Fluorescence Detection The c400 and c600 have powerful LEDs compatible with Cy5, Cy3 and Cy2 or similar dyes. Carry out multiplex blots detecting 2 or 3 different proteins using 2 or 3 different colors. Cy5 and Cy3 Images 120 micron resolution, less...

Blue Light Imaging An alternative to ethidium bromide, less-harmful ‘Safe’ dyes can be imaged with the EPI Blue LEDs standard in the system. SYBR® Safe, SYBR® Gold, SYBR® Green Protein Analysis White Light Imaging Protein gels stained with Coomassie blue or silver stain can easily be imaged using the visible light application. Coomassie Blue, Silver Stain DNA Detection with Ethidium Bromide With a dual-wavelength 302 nm and 365 nm UV transilluminator, images of ethidium bromide-stained DNA gels can be captured in a fraction of a second. An interlock switch prevents accidental exposure to UV....

Choose the system that suits your needs c600 Ultimate Western System Infrared Imaging System Visible Fluorescent Western System Darkroom Eliminator IR Fluorescence Visible Fluorescence Chemiluminescence UV Fluorescence Visible Imaging Blue Excited DNA Dyes IR Fluorescence Chemiluminescence UV Fluorescence Visible Imaging Blue Excited DNA Dyes Visible Fluorescence Chemiluminescence UV Fluorescence Visible Imaging Blue Excited DNA Dyes Chemiluminescence UV Fluorescence Visible Imaging Blue Excited DNA Dyes cSeries Specifications 7 Position Filter Wheel Visible Fluorescent Westerns Field of View...