BD AbSeq Immune Discovery Panel ® High-dimensional protein discovery tool for single-cell studies

Uncover up to 30 immune markers in a single experiment Performing protein discovery experiments can be challenging. From reagent handling and marker selection to ensuring accurate execution of all the necessary steps, probing samples for tens of protein markers in a single experiment is no trivial task. Introducing the BD® AbSeq Immune Discovery Panel (IDP), a simple tool that will enable you to uncover up to 30 different immune markers in a single experiment. The IDP consists of 30 different specificities against major human immune markers combined in a single tube. We developed the IDP by ensuring...

ONE-TUBE CONVENIENCE 30 pre-titrated antibodies against major human immune markers in a single tube Lyophilized format; simply reconstitute to stain samples Accompanied by comprehensive performance test data A great backbone panel that allows easy addition of more antibodies of interest MULTIOMICS ENABLED GREAT VALUE Manage your sequencing costs Works along with RNA and multiplexing assays 30 pre-titrated antibodies Specificity Steps for reconstituting the lyophilized IDP and staining cells 1. Remove the IDP tube from the foil bag and bring up to room temperature for 5 minutes. 7. Add 3 mL BD...

Reliable performance Ensuring optimal performance of antibodies when multiple antibodies are brought together in a panel is critical for immune discovery experiments. Keeping this in mind, we tested the performance of the individual 30 specificities included in the IDP against PBMCs from multiple donors. Our results demonstrate that all 30 specificities in the panel reliably detect their respective, individual targets. Figure 2. Performance of all 30 AbSeq specificities included in the IDP PBMC were activated and prepared as described in Figure 1. After staining, cells were captured on the BD...

Flexibility to add additional specificities of interest Figure 3A While the IDP provides the ideal backbone panel to quickly probe major immune markers of interest, we recognize there will be additional markers that you would like to include alongside IDP. The IDP is designed to accommodate additional AbSeq specificities and our results demonstrate that adding more BD AbSeq Antibodies does not impact the performance of the IDP or the added antibodies. We specifically chose three distinct specificities to demonstrate the above capability: CD38—a commonly expressed antigen; CD45RO —to test if this...

Multiomics and multiplexing enabled Perform RNA analyses and combine multiple samples with the IDP Performing multiomic analyses (RNA + protein) at the single-cell level can reveal deeper insights about your samples. In addition, performing such multiomic analyses on multiple samples together in a single experiment can reduce costs and most importantly help avoid inter-experimental batch effects. The IDP was developed to work seamlessly with BD Rhapsody RNA Assays and Multiplexing Kits. Figure 4A mRNA+IDP driven UMAP and Phenograph clustering mRNA driven UMAP and Phenograph clustering Subset...

The BD AbSeq Immune Discovery Panel is part of the broad BD portfolio of reagents, instruments and software designed to support your single-cell research. BD Rhapsody Express System Pool up to 12 samples to minimize batch effects and reduce time to discovery and experimental costs Single-cell capture, bead-based mRNA isolation and bead retrieval BD Rhapsody System Single-cell capture, bead-based mRNA isolation and bead retrieval with imaging and cell workflow QC BD Rhapsody™ Whole Transcriptome Analysis Kit BD Rhapsody™ Targeted Single-Cell RNAseq Panels BD AbSeq Single-Vial Reagents BD AbSeq...

Ordering Information Panel Description BD® AbSeq Immune Discovery Panel To request a quote or place an order, email [email protected] or contact your local BD sales representative. Suggested Companion Products Description BD Rhapsody ™ Single-Cell Analysis System BD Rhapsody ™ Express Single-Cell Analysis System BD Rhapsody ™ Whole Transcriptome Analysis Amplification Kit BD Rhapsody ™ Targeted mRNA Kit BD® Human Single-Cell Multiplexing Kit BD Pharmingen™ Human BD Fc Block™ Buffer Solution BD Rhapsody ™ Immune Response Panel BD Pharmingen™ Stain Buffer (FBS) For Research Use Only. Not...